Western Blot (WB)
(Western blot analysis of extracts from mouse heart tissueusing Myosin Light Chain 2 (Phospho-Ser18) antibody.)
Rabbit MRLC2 Polyclonal Antibody | anti-MRLC2 antibody
MRLC2 Antibody (Ser18)
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
MRLC2; Polyclonal Antibody; MRLC2 Antibody (Ser18); Rabbit Polyclonal MRLC2 Antibody (Ser18); Polyclonal MRLC2 antibody; Anti-MRLC2 antibody; ML12B antibody; MLC-2 antibody; MLC-2A antibody; MLC-B antibody; MLC20 antibody; MRLC2 antibody; MYL12B antibody; MYLC2B antibody; Myosin regulatory light chain 12B antibody; myosin regulatory light chain 2 antibody; Myosin regulatory light chain 2-B smooth muscle isoform antibody; Myosin regulatory light chain 20 kDa antibody; Myosin regulatory light chain MRLC2 antibody; myosin light chain 12B regulatory antibody; SHUJUN-1 antibody; anti-MRLC2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
MRLC2 antibody (Ser18) detects endogenous levels of Myosin LightChain 2 only when phosphorylated at serine 18
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-MRLC2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
MRLC2 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human MRLC2 around the phosphorylation site of serine 18 (A-T-SP-N-V) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts from mouse heart tissueusing Myosin Light Chain 2 (Phospho-Ser18) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse heart tissueusing Myosin Light Chain 2 (Phospho-Ser18) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MRLC2 antibody (Ser18))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MRLC2 antibody (Ser18))
Related Product Information for anti-MRLC2 antibody
The activity of nonmuscle myosin II is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments.
Product Categories/Family for anti-MRLC2 antibody
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Product Notes
The MRLC2 (Catalog #AAA5313557) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MRLC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the MRLC2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRLC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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