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Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1) using a 12% SDS PAGE gel and PSME3 antibody at a dilution of 1:2000)

Rabbit PSME3 Polyclonal Antibody | anti-PSME3 antibody

PSME3 Antibody

Gene Names
PSME3; Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
Applications
Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
PSME3; Polyclonal Antibody; PSME3 Antibody; Affinity Purified Rabbit Polyclonal PSME3 Antibody; Polyclonal PSME3 antibody; Anti-PSME3 antibody; 11S regulator complex subunit gamma antibody; PA28G antibody; 11S regulator complex gamma subunit antibody; PSME 3 antibody; REG-GAMMA antibody; Ki antibody; proteasome activator 28-gamma antibody; PSME3 antibody; PSME-3; proteasome activator 28 subunit gamma antibody; PA28-gamma antibody; PSME 3; PA28gamma antibody; PSME-3 antibody; Ki nuclear autoantigen antibody; anti-PSME3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.65 mg/ml (varies by lot)
Sequence Length
254
Applicable Applications for anti-PSME3 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
Immunogen
PSME3 antibody was raised in rabbit using a recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 254 of PSME3
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1) using a 12% SDS PAGE gel and PSME3 antibody at a dilution of 1:2000)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1) using a 12% SDS PAGE gel and PSME3 antibody at a dilution of 1:2000)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded CA922 xenograft using PSME3 antibody at a dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded CA922 xenograft using PSME3 antibody at a dilution of 1:100)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: PC-12) using a 12% SDS PAGE gel and PSME3 antibody at a dilution of 1:2000)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: PC-12) using a 12% SDS PAGE gel and PSME3 antibody at a dilution of 1:2000)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSME3 antibody at 1:500 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSME3 antibody at 1:500 dilution.)
Related Product Information for anti-PSME3 antibody
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator.
Product Categories/Family for anti-PSME3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
30 kDa
NCBI Official Full Name
PSME3
NCBI Official Synonym Full Names
proteasome activator subunit 3
NCBI Official Symbol
PSME3
NCBI Official Synonym Symbols
Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
NCBI Protein Information
proteasome activator complex subunit 3
Protein Family

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

Research Articles on PSME3

Similar Products

Product Notes

The PSME3 (Catalog #AAA5312108) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PSME3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:100-1:1000 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the PSME3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSME3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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