PSME3 recombinant protein

PSME3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121763

• Gene Names: PSME3; Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
• Purity: >90% as determined by SDS-PAGE
• Synonyms: PSME3; PSME3 Protein; Human; Recombinant (His Tag); Human PSME3/PA28-gamma Protein (His Tag); HEL-S-283 Protein; Ki Protein; PA28-gamma Protein; PA28G Protein; PA28gamma Protein; REG-GAMMA Protein; proteasome (prosome; macropain) activator subunit 3 (PA28 gamma; Ki); PSME3 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 200mM NaCl, 40% Glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr254

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human PSME3 (P61289-1)(Met1-Tyr254) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PSME3 recombinant protein

Background: PSME3, also known as PA28-gamma, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 2S core and a 19S regulator. The 2S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 1 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME3 gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring.

References

Albertsen HM. et al., 1994, Nat Genet. 7 (4): 472-9. Zhang Zhuo. et al., 2008, EMBO J. 27 (6): 852-64. Coux O. et al., 1996, Annu Rev Biochem. 65: 801-47.

NCBI and Uniprot Product Information

• NCBI GI #: 388556516
• NCBI GeneID: 10197
• NCBI Accession #: NP_001253974.1
• NCBI GenBank Nucleotide #: NM_001267045.1
• UniProt Accession #: P61289; O35563; P97373; Q12920; Q13172; Q9BQD9; A8K9A3
• Molecular Weight: 29,506 Da
• NCBI Official Full Name: proteasome activator complex subunit 3 isoform 3
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
• NCBI Official Symbol: PSME3
• NCBI Official Synonym Symbols: Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
• NCBI Protein Information: proteasome activator complex subunit 3; Ki antigen; REG gamma-3; PA28 gamma variant 5; Ki nuclear autoantigen; proteasome activator 28-gamma; 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; epididymis secretory protein Li 283; pr
• UniProt Protein Name: Proteasome activator complex subunit 3
• Protein Family: Proteasome activator
• UniProt Gene Name: PSME3
• UniProt Synonym Gene Names: REG-gamma; PA28g; PA28gamma
• UniProt Entry Name: PSME3_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

Uniprot Description

PSME3: a protein implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Homohexamer. Significantly elevated in colorectal cancer patients compared with healthy donors and patients with benign bowel disease. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; proteasome complex; proteasome activator complex; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; p53 binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis