Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed A549, using GRAP antibody at 1:500 dilution.)
Rabbit GRAP Polyclonal Antibody | anti-GRAP antibody
GRAP Antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
GRAP; Polyclonal Antibody; GRAP Antibody; Affinity Purified Rabbit Polyclonal GRAP Antibody; Polyclonal GRAP antibody; Anti-GRAP antibody; GRB2-related adapter protein antibody; growth factor receptor-bound protein 2-related adaptor protein antibody; GRAP antibody; MGC64880 antibody; GRB2-related adaptor protein antibody; anti-GRAP antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-GRAP antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
ICC: 1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Immunogen
GRAP antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 155 and 217 of GRAP
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed A549, using GRAP antibody at 1:500 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed A549, using GRAP antibody at 1:500 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A549) using GRAP antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A549) using GRAP antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded U373 xenograft using GRAP antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded U373 xenograft using GRAP antibody at a dilution of 1:100)
Related Product Information for anti-GRAP antibody
This gene encodes a member of the GRB2/Sem5/Drk family. This member functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain.
Product Categories/Family for anti-GRAP antibody
Similar Products
Product Notes
The GRAP (Catalog #AAA5311960) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GRAP can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:500 ICC: 1:100-1:500 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the GRAP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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