Rabbit Hsp27 Polyclonal Antibody | anti-Hsp27 antibody
Hsp27 Antibody
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Affinity Chromatography
Synonyms
Hsp27; Polyclonal Antibody; Hsp27 Antibody; Rabbit Polyclonal Hsp27 Antibody; Polyclonal Hsp27 antibody; anti-Hsp27 antibody; HSP28 antibody; HSP27 antibody; Hsp25 antibody; CMT2F antibody; Hsp 27 antibody; Hsp-27; DKFZp586P1322 antibody; HSPB1 antibody; Hsp-27 antibody; HS.76067 antibody; Hsp 27
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer, pH 7.2, containing 50% Glycerol, 1% BSA, 0.02% Thimerosal
Concentration
200 ug/ml (varies by lot)
Applicable Applications for anti-Hsp27 antibody
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ELISA: 1 ug/ml
IP: 5-10 ug/ml
ICC: 10-15 ug/ml
WB: 1-2 ug/ml
IP: 5-10 ug/ml
ICC: 10-15 ug/ml
WB: 1-2 ug/ml
Immunogen
Hsp27 antibody was raised in Rabbit using recombinant human Hsp27 as the immunogen.
Cross-Reactivity
Human, Monkey
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Related Product Information for anti-Hsp27 antibody
Human Hsp27, mouse Hsp25 and alphabeta-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.
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Product Notes
The Hsp27 (Catalog #AAA5311163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hsp27 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). ELISA: 1 ug/ml IP: 5-10 ug/ml ICC: 10-15 ug/ml WB: 1-2 ug/ml. Researchers should empirically determine the suitability of the Hsp27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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