Rabbit anti-Rat Hsp27 Polyclonal Antibody | anti-Hsp27 antibody

Hsp27 Antibody

Cat. Num. AAA840260

• Gene Names: Fas; lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Hsp27; Polyclonal Antibody; Hsp27 Antibody; HSPB1; CMT2F; DKFZp586P1322; HS.76067; HSP27; HSP28; Hsp25; anti-Hsp27 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 327

• Applicable Applications for anti-Hsp27 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects 27 kDa rat Hsp27. Rat kidney tissue lysate can be used as a positive control.
• Immunogen: Synthetic peptide surrounding amino acid 193 of rat Hsp2
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-Hsp27 antibody

Background: Hsp27 exhibits a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.

NCBI and Uniprot Product Information

• NCBI GI #: 226443049
• NCBI GeneID: 14102
• NCBI Accession #: NP_032013.2
• NCBI GenBank Nucleotide #: NM_007987.2
• UniProt Accession #: P25446; Q6GT31; Q9DCQ1
• Molecular Weight: 37,437 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas (TNF receptor superfamily member 6)
• NCBI Official Symbol: Fas
• NCBI Official Synonym Symbols: lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• Protein Family: Heat shock protein
• UniProt Gene Name: Fas
• UniProt Synonym Gene Names: Apt1; Tnfrsf6
• UniProt Entry Name: TNR6_MOUSE

Uniprot Description

FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Apoptosis; Receptor, cytokine; Cell surface

Cellular Component: extracellular space; neuron projection; cell surface; extracellular region; integral to membrane; CD95 death-inducing signaling complex; secretory granule; lipid raft; cell soma; membrane; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; plasma membrane; nucleus; sarcolemma; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; tumor necrosis factor receptor activity; protease binding; protein complex binding; kinase binding

Biological Process: renal system process; apoptosis; positive regulation of apoptosis; regulation of lymphocyte differentiation; response to toxin; response to glucocorticoid stimulus; positive regulation of caspase activity; signal transduction; regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; T cell homeostasis; immunoglobulin production; activated T cell apoptosis; protein homooligomerization; spleen development; regulation of myeloid cell differentiation; transformed cell apoptosis; positive regulation of protein homooligomerization; negative regulation of B cell activation; negative thymic T cell selection; regulation of cell proliferation; dendrite regeneration; B cell mediated immunity; inflammatory cell apoptosis; neuron apoptosis; induction of apoptosis via death domain receptors; regulation of gene expression; immune response; gene expression; negative regulation of apoptosis