Immunofluorescence (IF)
(Immunofluorescence analysis of ECA109 cells using KLF4 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Mouse KLF4 Monoclonal Antibody | anti-KLF4 antibody
KLF4 antibody
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
KLF4; Monoclonal Antibody; KLF4 antibody; Mouse monoclonal KLF4 antibody; Monoclonal KLF4 antibody; Anti-KLF4 antibody; KLF-4 antibody; KLF 4 antibody; Krueppel-like factor 4 antibody; EZF antibody; GKLF antibody; Gut enriched kruppel-like factor antibody; GkLF antibody; KLF 4; KLF-4; anti-KLF4 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1E6
Form/Format
Liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-KLF4 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:10,000
IF: 1:200-1:1000
IHC: 1:200-1:1000
WB: 1:200-1:1000
IF: 1:200-1:1000
IHC: 1:200-1:1000
WB: 1:200-1:1000
Immunogen
KLF4 antibody was raised in Mouse using a purified recombinant fragment of human KLF4 expressed in E Coli as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of ECA109 cells using KLF4 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Immunofluorescence (IF)
(Immunofluorescence analysis of ECA109 cells using KLF4 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Western Blot (WB)
(Western Blot showing KLF4 antibody used against HEK293 (1) and KLF4 (AA: 2-180)-hIgGFc transfected HEK293 (2) cell lysate.)
Western Blot (WB)
(Western Blot showing KLF4 antibody used against HEK293 (1) and KLF4 (AA: 2-180)-hIgGFc transfected HEK293 (2) cell lysate.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and lung cancer tissues (right) using KLF4 antibody with DAB staining)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and lung cancer tissues (right) using KLF4 antibody with DAB staining)
Related Product Information for anti-KLF4 antibody
Kruppel-like factor 4 (gut), endothelial Kruppel-like zinc finger protein. May act as a transcriptional activator. Binds the CACCC core sequence. May be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 is highly expressed in the epithelial cells of the skin and the gastrointestinal tract.
Product Categories/Family for anti-KLF4 antibody
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Product Notes
The KLF4 (Catalog #AAA5310905) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's KLF4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:10,000 IF: 1:200-1:1000 IHC: 1:200-1:1000 WB: 1:200-1:1000. Researchers should empirically determine the suitability of the KLF4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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