Glutamate Pyruvate Transaminase Active Protein | GPT active protein

Glutamate Pyruvate Transaminase protein

• Gene Names: GPT; AAT1; ALT1; GPT1
• Synonyms: Glutamate Pyruvate Transaminase; Glutamate Pyruvate Transaminase protein; Alanine aminotransferase protein; GPT active protein

• Host: Human
• Form/Format: Yellow-green powder. Lyophilized from 25mM Tris Chloride, pH 7.0 with 1mM EDTA, 1mM DTT, 0.05mM alpha-ketoglutarate, and 0.05mM pyridoxal-5-phosphate.
• Concentration: 100 units, lyophilized (varies by lot)
• Sequence Length: 496

• Source: Human Liver
• Protein Type: Native
• Biological Significance: This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis.
• Contaminants: ALP <0.1%; CK <0.1%; gGT <0.1%; GOT/AST 0.6%; LDH <0.1%
• Protein: 0.91 mg protein/mg solid (Coomasie)
• Activity: 33 U/mg solid
• Specific Activity: 36 U/mg protein
• Biohazard: non-reactive for HIV-1/HCV/HBV by NAT, HBsAg, HCV Ab, HIV 1&2 Ab, and RPR by currently approved FDA methods
• Preparation and Storage: Ships ambient or refrigerated. Upon receipt store at -20°C

Related Product Information for GPT active protein

Purified native Human Glutamate Pyruvate Transaminase protein

Product Categories/Family for GPT active protein

Proteases; Inhibitors; & Enzymes; Native Protein

NCBI and Uniprot Product Information

• NCBI GI #: 1763096
• NCBI GeneID: 2875
• Molecular Weight: 57 kDa
• NCBI Official Full Name: glutamate pyruvate transaminase
• NCBI Official Synonym Full Names: glutamic-pyruvate transaminase (alanine aminotransferase)
• NCBI Official Symbol: GPT
• NCBI Official Synonym Symbols: AAT1; ALT1; GPT1
• NCBI Protein Information: alanine aminotransferase 1
• UniProt Protein Name: Alanine aminotransferase 1
• Protein Family: Alanine aminotransferase
• UniProt Gene Name: GPT
• UniProt Synonym Gene Names: AAT1; GPT1; ALT1; GPT 1
• UniProt Entry Name: ALAT1_HUMAN

NCBI Description

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

Uniprot Description

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; EC 2.6.1.2

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine catabolic process