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Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Collagen XX alpha 1 antibody)

Rabbit anti-Human, Mouse Collagen XX alpha 1 Polyclonal Antibody

Collagen XX alpha 1 antibody

Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
Collagen XX alpha 1; Polyclonal Antibody; Collagen XX alpha 1 antibody; Polyclonal Collagen XX alpha 1; Anti-Collagen XX alpha 1; Collagen type XX alpha 1; COL10A1; COL10A 1; COL10A-1; anti-Collagen XX alpha 1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Collagen XX alpha 1 antibody detects endogenous levels of total Collagen XX alpha1 protein
Form/Format
Rabbit IgG supplied as a liquid in PBS, pH 7.4, with 150mM NaCl, 0.02% NaN3, and 50% Glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-Collagen XX alpha 1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:20000
Biological Significance
Collagen XX alpha 1 is the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification.
Immunogen
synthesized peptide derived from C-terminal of human Collagen XXalpha1.
Preparation and Storage
Store in small aliquots at -20 degree C. Avoid repeated freeze/thaw cycles

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Collagen XX alpha 1 antibody)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Collagen XX alpha 1 antibody)

Immunofluorescent (IF)

(Immunofluorescent staining using Collagen XX alpha 1 antibody (MBS5303001) Immunofluorescence analysis of NIH/3T3 cells using Collagen XX alpha 1 antibody)

Immunofluorescent (IF) (Immunofluorescent staining using Collagen XX alpha 1 antibody (MBS5303001) Immunofluorescence analysis of NIH/3T3 cells using Collagen XX alpha 1 antibody)

Western blot (WB)

(Western blot analysis using Collagen XX alpha 1 antibody (MBS5303001) Western blot analysis of extracts from HUVEC cells using Collagen XX alpha 1 antibody)

Western blot (WB) (Western blot analysis using Collagen XX alpha 1 antibody (MBS5303001) Western blot analysis of extracts from HUVEC cells using Collagen XX alpha 1 antibody)
Related Product Information for anti-Collagen XX alpha 1 antibody
Rabbit polyclonal Collagen XX alpha 1 antibody
Product Categories/Family for anti-Collagen XX alpha 1 antibody

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Product Notes

The Collagen XX alpha 1 (Catalog #AAA5303001) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen XX alpha 1 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen XX alpha 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:100-1:500 ELISA: 1:20000. Researchers should empirically determine the suitability of the Collagen XX alpha 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen XX alpha 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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