Testing Data
(Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue))
Mouse His Tag Monoclonal Antibody
Anti-His Tag (HIS.H8) Monoclonal Antibody
Applications
Dot Blot, ELISA, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Protein A affinity chromatography from mouse ascites fluid
Synonyms
His Tag; Monoclonal Antibody; Anti-His Tag (HIS.H8) Monoclonal Antibody; Anti-His Tag; anti-His Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
HIS.H8
Specificity
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Purity/Purification
Protein A affinity chromatography from mouse ascites fluid
Form/Format
1 mg/ml in 10mM PBS, pH 7.2, with 0.05% NaN3
Applicable Applications for anti-His Tag antibody
Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB)
Application Notes
WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature).
IS: 1:500-2000 dilution
IP: 1:500-200 dilution
For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilutions by titration test
IS: 1:500-2000 dilution
IP: 1:500-200 dilution
For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilutions by titration test
Immunogen
HHHHHH (6x His) synthetic peptide conjugated to KLH
Sensitivity
WB (with ECL): 1-2ng of His-tagged fusion proteins
Preparation and Storage
Store at -20 degree C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4 degree C for several days to weeks.
Testing Data
(Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue))
Testing Data
(Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue))
Testing Data #2
(Fig. 02 Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL))
Testing Data #2
(Fig. 02 Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL))
Testing Data #3
(Fig. 03 Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2))
Testing Data #3
(Fig. 03 Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2))
Product Categories/Family for anti-His Tag antibody
Similar Products
Product Notes
The His Tag (Catalog #AAA525001) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's His Tag can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB). WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution IP: 1:500-200 dilution For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilutions by titration test. Researchers should empirically determine the suitability of the His Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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