PRMT7 recombinant protein

PRMT7 Protein

Cat. Num. AAA517412

• Applications: Western Blot
• Synonyms: PRMT7; PRMT7 Protein; FLJ10640; KIAA1933; PRMT7 recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

• Applicable Applications for PRMT7 recombinant protein: Western Blot (WB)
• Tag: GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for PRMT7 recombinant protein

Recombinant full-length human PRMT7 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The PRMT7 gene accession number is BC000146.
PRMT7 (protein arginine methyltransferase 7), an arginine methyltransferase that exhibited type III enzymatic activity, catalyzing the formation of omega-NG-monomethylarginine residues. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency.

Product Categories/Family for PRMT7 recombinant protein

Acetyl & Methyltransferase Proteins; Apoptosis/Autophagy; Cancer; Cell Cycle; Neurobiology

References

1. Miranda TB, et al: PRMT7 is a member of the protein arginine methyltransferase family with a distinct substrate specificity. J Biol Chem. 279(22):22902-7, 2004.
2. Lee JH, et al: PRMT7, a new protein arginine methyltransferase that synthesizes symmetric dimethylarginine. J Biol Chem. 280(5):3656-64, 2005.
3. http://www.uniprot.org/uniprot/Q9NVM4

NCBI and Uniprot Product Information

• NCBI GI #: 12652788
• NCBI GeneID: 54496
• Molecular Weight: 63,806 Da
• NCBI Official Full Name: Homo sapiens protein arginine methyltransferase 7, mRNA
• NCBI Official Synonym Full Names: protein arginine methyltransferase 7
• NCBI Official Symbol: PRMT7
• NCBI Protein Information: protein arginine N-methyltransferase 7

NCBI Description

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM, Mar 2008]