FGF10 recombinant protein

FGF10

Cat. Num. AAA517002

• Applications: Cell Assay
• Synonyms: FGF10; FGF-10; FGFA; Keratinocyte growth factor-2 Recombinant Human Fibroblast Growth Factor 10 (FGF10); FGF10 recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from 0.2 um filtered solution in PBS (pH7.0) and 1mM DTT

• Applicable Applications for FGF10 recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: Storage The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2o -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.)

SDS-Page

(Purity: Recombinant Human FGF10 resolved on a 15% SDS_PAGE gel under reducing conditions and stained with Coomassie Blue G-250.)

Related Product Information for FGF10 recombinant protein

Recombinant Human FGF10 was expressed in E Coli cells. The protein accession number is O15520
FGF-10 is involved in the initial budding as well as the continuous outgrowth of vertebrate limbs. FGF-10 mRNA is expressed preferentially in neurons but not in glial cells and may have a distinct role in the brain. Human FGF-10 is mitogenic for fetal rat keratinizing epidermal cells but not for NIH 3T3 cells. Recombinant FGF-10 induces the proliferation of human urothelial cells in vitro and induces the proliferation of transitional epithelium. FGF-10 is secreted by cultured mouse pre-adipocytes, prevention of FGF-10 signaling inhibits subsequent differentiation. The ability of embryonic fibroblasts derived from FGF-10 knock-out mice to differentiate into adipocytes is also impaired.

Product Categories/Family for FGF10 recombinant protein

Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4758360
• NCBI GeneID: 2255
• NCBI Accession #: NP_004456.1
• NCBI GenBank Nucleotide #: NM_004465.1
• UniProt Accession #: O15520; Q6FHT6; Q96P59
• Molecular Weight: 23,436 Da
• NCBI Official Full Name: fibroblast growth factor 10
• NCBI Official Synonym Full Names: fibroblast growth factor 10
• NCBI Official Symbol: FGF10
• NCBI Protein Information: fibroblast growth factor 10; FGF-10; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
• UniProt Protein Name: Fibroblast growth factor 10
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF10
• UniProt Synonym Gene Names: FGF-10
• UniProt Entry Name: FGF10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF10: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing. Interacts with FGFR1 and FGFR2. Interacts with FGFBP1. Belongs to the heparin-binding growth factors family.

Protein type: Cell development/differentiation; Cytokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5p13-p12

Cellular Component: extracellular matrix; extracellular space; cell surface; plasma membrane; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; growth factor activity; type 2 fibroblast growth factor receptor binding; fibroblast growth factor receptor binding; chemoattractant activity

Biological Process: nerve growth factor receptor signaling pathway; salivary gland development; urothelial cell proliferation; somatic stem cell maintenance; activation of MAPK activity; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of transcription, DNA-dependent; muscle cell fate commitment; response to lipopolysaccharide; regulation of saliva secretion; embryonic pattern specification; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; positive regulation of fibroblast proliferation; epithelial cell proliferation; positive chemotaxis; embryonic digestive tract morphogenesis; induction of an organ; mesonephros development; embryonic genitalia morphogenesis; positive regulation of keratinocyte migration; spleen development; positive regulation of DNA repair; fibroblast growth factor receptor signaling pathway; positive regulation of urothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; determination of left/right symmetry; metanephros development; positive regulation of epithelial cell proliferation; wound healing; radial glial cell differentiation; positive regulation of mitotic cell cycle; response to estradiol stimulus; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; establishment of mitotic spindle orientation; positive regulation of lymphocyte proliferation; tissue regeneration; male genitalia morphogenesis; pancreas development; thyroid gland development; lacrimal gland development; angiogenesis; otic vesicle formation; female genitalia morphogenesis; positive regulation of Notch signaling pathway; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; hair follicle morphogenesis; thymus development; keratinocyte proliferation; regulation of activin receptor signaling pathway; embryonic camera-type eye development; odontogenesis of dentine-containing teeth; limb bud formation; pituitary gland development; positive regulation of ATPase activity; actin cytoskeleton reorganization; white fat cell differentiation; insulin receptor signaling pathway; innate immune response; blood vessel remodeling; positive regulation of Ras protein signal transduction; positive regulation of DNA replication; regulation of smoothened signaling pathway

Disease: Lacrimoauriculodentodigital Syndrome; Aplasia Of Lacrimal And Salivary Glands