Rabbit anti-All mammals Tyrosine Hydroxylase Polyclonal Antibody

Anti-Tyrosine Hydroxylase

Cat. Num. AAA515996

• Reactivity: All mammals
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Affinity chromatography
• Synonyms: Tyrosine Hydroxylase; Polyclonal Antibody; Anti-Tyrosine Hydroxylase; anti-Tyrosine Hydroxylase antibody

• Host: Rabbit
• Reactivity: All mammals
• Clonality: Polyclonal
• Isotype: Rabbit, IgG
• Specificity: Recognizes the Tyrosine Hydroxylase (TH) protein
• Purity/Purification: Affinity chromatography
• Form/Format: 10mM HEPES (pH7.5), 150mM NaCl, 100 ug per ml BSA and 50% glycerol.

• Applicable Applications for anti-Tyrosine Hydroxylase antibody: Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Immunogen: SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.
• Preparation and Storage: Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.; 1 year at -20 degree C from date of shipment

Western Blot (WB)

(Sample Data: Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k Tyrosine Hydroxylase protein.)

Related Product Information for anti-Tyrosine Hydroxylase antibody

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Tyrosine Hydroxylase antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).

Product Categories/Family for anti-Tyrosine Hydroxylase antibody

Primary Antibodies; Neurobiology

References

1. Dunkley PR, Bobrovskaya L, et al. Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. 2001.
2. Salvatore MF, Waymire JC, Haycock JW, Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360. 2001.
3. Witkovsky P, Gabriel R, Haycock JW, Meller E, Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116. 2000.
4. Xu ZQ, Lew JY, Harada K, et al, Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site-and phosphorylation state-specific antibodies. Neurosci 82:727-738. 1998.
5. Zhu MY, Klimek V, Haycock JW, Ordway GA, Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. 2000.
6. Zhu MY, Klimek V, Dilley GE, et al, Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286. 1999.