PDE4B recombinant protein

PDE4B, Active

Cat. Num. AAA515631

• Gene Names: PDE4B; DPDE4; PDEIVB
• Applications: PDE Assay
• Synonyms: PDE4B; Active; PDE4B recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: 305-end
• Sequence Length: 4264

• Applicable Applications for PDE4B recombinant protein: PDE Assay
• Species: Human
• Tag: GST tag
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for PDE4B recombinant protein

Description: Recombinant human PDE4B (305-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE4B is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and has homology to the 'dunce' gene of Drosophila. The 'dunce' gene is one of several genes critical for normal learning and memory in the fly. Altered activity of PDE4B has been associated with schizophrenia and bipolar affective disorder. DISC1 interacts with the UCR2 domain of PDE4B and elevation of cellular cAMP leads to dissociation of PDE4B from DISC1 and in increase in PDE4B activity (1). PDE4B gene itself does not link to major depressive disorder (MDD) but elevated mRNA levels of PDE4B have been implicated in the pathophysiology of MDD (2).

Product Categories/Family for PDE4B recombinant protein

Enzymes; Active Phosphodiesterases

References

1. Millar J K, et al: DISC1 and PDE4B are interacting genetic factors in schizophrenia that regulate cAMP signaling. Science 310: 1187-1191, 2005. 2. Numata S, et al: Gene expression and association analyses of the phosphodiesterase 4B (PDE4B) gene in major depressive disorder in the Japanese population. Am J Med Genet B Neuropsychiatr Genet. 2009 Jun 5;150B (4):527-34.

NCBI and Uniprot Product Information

• NCBI GI #: 82799480
• NCBI GeneID: 5142
• NCBI Accession #: NM_002600.3
• NCBI GenBank Nucleotide #: NM_002600
• UniProt Accession #: Q07343; O15443; Q13945; Q5TEK4; Q5TEK5; Q5TEK6; A5YW33
• Molecular Weight: ~78 kDa
• NCBI Official Full Name: Homo sapiens phosphodiesterase 4B (PDE4B), transcript variant a, mRNA
• NCBI Official Synonym Full Names: phosphodiesterase 4B
• NCBI Official Symbol: PDE4B
• NCBI Official Synonym Symbols: DPDE4; PDEIVB
• NCBI Protein Information: cAMP-specific 3',5'-cyclic phosphodiesterase 4B
• UniProt Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4B
• Protein Family: cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE4B
• UniProt Synonym Gene Names: DPDE4

NCBI Description

This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

Uniprot Description

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.