COT recombinant protein

COT, Active

Cat. Num. AAA515539

• Gene Names: MAP3K8; COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
• Applications: Western Blot
• Synonyms: COT; Active; COT recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: 30-397
• Sequence Length: 3013

• Applicable Applications for COT recombinant protein: Kinase Assay, Western Blot (WB)
• Species: Human
• Tag: GST tag
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for COT recombinant protein

Description: Recombinant human COT (30-397) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates IkappaB kinases and induces the nuclear production of NF-kappaB. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner (1). Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of Cot results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels (2).

Product Categories/Family for COT recombinant protein

Enzymes; Active Kinases

References

1. Channavajhala, P L. et al: Identification of a novel human kinase supporter of Ras (hKSR-2) that functions as a negative regulator of Cot (Tpl2) signaling. J. Biol. Chem. 278: 47089-47097, 2003. 2. Rodr-guez C. et al: TRAF6 and Src kinase activity regulates Cot activation by IL-1. Cell Signal. 2006 Sep;18 (9):1376-85.

NCBI and Uniprot Product Information

• NCBI GI #: 346644791
• NCBI GeneID: 1326
• NCBI Accession #: NM_005204.3
• NCBI GenBank Nucleotide #: NM_005204
• UniProt Accession #: P41279; Q14275; Q5T855; Q9HC81; A8K2Q5; D3DRX1
• Molecular Weight: ~70 kDa
• NCBI Official Full Name: Homo sapiens mitogen-activated protein kinase kinase kinase 8 (MAP3K8), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 8
• NCBI Official Symbol: MAP3K8
• NCBI Official Synonym Symbols: COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 8
• UniProt Gene Name: MAP3K8
• UniProt Synonym Gene Names: COT; ESTF; TPL-2

NCBI Description

This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

Cot: an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 2.7.11.25; Kinase, protein; Oncoprotein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE-Unique family

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: cytoplasm; cytosol

Molecular Function: MAP kinase kinase kinase activity; protein binding; protein serine/threonine kinase activity

Biological Process: activation of protein kinase activity; protein amino acid phosphorylation; regulation of apoptosis; regulation of mitotic cell cycle; stress-activated MAPK cascade; stress-activated protein kinase signaling pathway; T cell costimulation

Disease: Lung Cancer