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SDS-PAGE

UBE2A recombinant protein

UBE2A recombinant protein

Gene Names
UBE2A; UBC2; HHR6A; MRXSN; RAD6A; MRXS30
Applications
Western Blot
Synonyms
UBE2A; UBE2A recombinant protein; HHR6A; MRXS30; MRXSN; RAD6A; UBC2; UBE2A protein
Ordering
For Research Use Only!
Host
E Coli
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Sequence Length
1878
Applicable Applications for UBE2A recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
E.coli
Source Note
Recombinant full length human UBE2A was expressed in E. coli cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

SDS-PAGE
Related Product Information for UBE2A recombinant protein
Recombinant full length human UBE2A was expressed in E. coli cells using an N-terminal His tag.

Scientific Background: UBE2A or ubiquitin-conjugating enzyme E2A is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes (1). RAD18 and RAD5 play a central role in mediating physical contacts between the members of the RAD6 pathway. UBE2A is specifically important during chronic low dose ultraviolet exposure to prevent counterproductive DNA checkpoint activation and allow cells to proliferate normally (2). Loss of the RAD6 error-free PRR pathway results in DNA damage checkpoint-induced G2 arrest in CLUV-exposed cells.
Product Categories/Family for UBE2A recombinant protein
References
1. Ulrich, H. D. et.al: Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair. EMBO J. 19: 3388-3397, 2000.
2. Hishida, T. et.al: RAD6-RAD18-RAD5-pathway-dependent tolerance to chronic low-dose ultraviolet light. Nature 457: 612-615, 2009.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~17 kDa
NCBI Official Full Name
Homo sapiens ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2A
NCBI Official Symbol
UBE2A
NCBI Official Synonym Symbols
UBC2; HHR6A; MRXSN; RAD6A; MRXS30
NCBI Protein Information
ubiquitin-conjugating enzyme E2 A; RAD6 homolog A; ubiquitin-protein ligase A; ubiquitin carrier protein A
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 A
UniProt Gene Name
UBE2A
UniProt Synonym Gene Names
RAD6A; HR6A; hHR6A
UniProt Entry Name
UBE2A_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Ref.7 Ref.9

Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with RAD18 and WAC. Ref.11

Post-translational modification: Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B.

Involvement in disease: Mental retardation, X-linked, syndromic, Nascimento-type (MRXSN) [MIM:300860]: A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSN features include dysmorphic facies, hirsutism, skin and nails abnormalities, obesity, speech anomalies and seizures.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8 Ref.13

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.

Research Articles on UBE2A

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Product Notes

The UBE2A ube2a (Catalog #AAA515327) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His tag!!Expression System||E.coli!!Source Note||Recombinant full length human UBE2A was expressed in E. coli cells. AAA Biotech's UBE2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the UBE2A ube2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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