14-3-3 epsilon recombinant protein

14-3-3 epsilon recombinant protein

Cat. Num. AAA515294

• Gene Names: YWHAE; MDS; MDCR; KCIP-1; 14-3-3E
• Applications: Western Blot
• Synonyms: 14-3-3 epsilon; 14-3-3 epsilon recombinant protein; YWHAE; MDS; MDCR; KCIP-1; 14-3-3E; FLJ45465; 14-3-3epsilon Protein; 14-3-3e Protein

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 1827

• Applicable Applications for 14-3-3 epsilon recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: tag-free
• Expression System: E.coli
• Source Note: Recombinant full-length human tag-free 14-3-3epsilon was expressed in E. coli.
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles

SDS-PAGE

Related Product Information for 14-3-3 epsilon recombinant protein

Recombinant full-length human tag-free 14-3-3epsilon was expressed in E. coli.

Scientific Background: 14-3-3thetau, also known as tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein is a member of the 14-3-3 family. 14-3-3thetau has been reported to be involved in heat shock transcription factor 1 (HSF1) and extracellular signal regulated protein kinase (ERK) pathways (1). 14-3-3thetau interaction with the deubiquitinating enzyme UBPY, also known as USP8, regulates cargo sorting and membrane traffic at early endosomes. Association of 14-3-3thetau, HSF1 with ERK during heat shock may modulate the amplitude of the response and control the termination of HSP expression on resumption of growth conditions (2).

Product Categories/Family for 14-3-3 epsilon recombinant protein

Cell Stress & Chaperone Proteins; Signaling Proteins - Cell Stress & Chaperone Proteins

References

1.Wang, X. et al: Interactions between extracellular signal-regulated protein kinase 1, 14-3-3epsilon, and heat shock factor 1 during stress.J. Biol. Chem. 2004; 279(47):49460-9.
2.Mizuno, E. et al: 14-3-3-dependent inhibition of the deubiquitinating activity of UBPY and its cancellation in the M phase.Exp Cell Res. 2007; 313(16):3624-34.

NCBI and Uniprot Product Information

• NCBI GI #: 195546907
• NCBI GeneID: 7531
• NCBI Accession #: NP_006752.1
• NCBI GenBank Nucleotide #: NM_006761
• UniProt Accession #: P62258; P29360; P42655; Q4VJB6; Q53XZ5; Q63631; Q7M4R4; B3KY71; D3DTH5
• Molecular Weight: ~30kDa
• NCBI Official Full Name: Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• NCBI Official Symbol: YWHAE
• NCBI Official Synonym Symbols: MDS; MDCR; KCIP-1; 14-3-3E
• NCBI Protein Information: 14-3-3 protein epsilon; 14-3-3 epsilon; protein kinase C inhibitor protein-1; mitochondrial import stimulation factor L subunit; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
• UniProt Protein Name: 14-3-3 protein epsilon
• UniProt Gene Name: YWHAE
• UniProt Synonym Gene Names: 14-3-3E
• UniProt Entry Name: 1433E_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Subunit structure: Homodimer. Heterodimerizes with YWHAZ. Interacts with NDEL1, ARHGEF28 and TIAM2

By similarity. Interacts with HCV core protein. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with GAB2. Interacts with phosphorylated GRB10. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2. Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.22 Ref.25 Ref.30

Subcellular location: Cytoplasm

By similarity. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.17 Ref.21

Sequence similarities: Belongs to the 14-3-3 family.