Human C-type lectin domain family 7, member A ELISA Kit | CLEC7A elisa kit

Human C-type lectin domain family 7 member A, CLEC7A ELISA Kit

Cat. Num. AAA931358

• Gene Names: CLEC7A; BGR; CANDF4; DECTIN1; CLECSF12
• Reactivity: Human
• Synonyms: C-type lectin domain family 7; member A; Human C-type lectin domain family 7 member A; CLEC7A ELISA Kit; Human C-type lectin domain family 7 member A (CLEC7A) ELISA kit; BGR; CANDF4; CLECSF12; DECTIN1; C-type (calcium dependent; carbohydrate-recognition domain) lectin; superfamily member 12; beta-glucan receptor; dectin-1; dendritic cell-associated C-type lectin 1; dend; CLEC7A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CLEC7A. No significant cross-reactivity or interference between Human CLEC7A and analogues was observed.
• Sequence Length: 201

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: 7.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 3: Immunity
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLEC7A elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLEC7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLEC7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLEC7A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLEC7A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 13384604
• NCBI GeneID: 64581
• NCBI Accession #: NP_072092.2
• NCBI GenBank Nucleotide #: NM_022570.4
• UniProt Accession #: Q9BXN2; Q6IPS7; Q96D32; Q96DR9; Q96LD3; Q96PA4; Q96PA5; Q96PA6; B2R861; B7Z494; B7Z5A9; B7Z5B9
• Molecular Weight: 27,627 Da
• NCBI Official Full Name: C-type lectin domain family 7 member A isoform b
• NCBI Official Synonym Full Names: C-type lectin domain family 7, member A
• NCBI Official Symbol: CLEC7A
• NCBI Official Synonym Symbols: BGR; CANDF4; DECTIN1; CLECSF12
• NCBI Protein Information: C-type lectin domain family 7 member A; dectin-1; beta-glucan receptor; lectin-like receptor 1; DC-associated C-type lectin 1; C-type lectin superfamily member 12; dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12
• UniProt Protein Name: C-type lectin domain family 7 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC7A
• UniProt Synonym Gene Names: BGR; CLECSF12; DECTIN1; UNQ539/PRO1082; DC-associated C-type lectin 1; Dectin-1
• UniProt Entry Name: CLC7A_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Uniprot Description

dectin-1: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Up-regulated during differentiation from monocytes into dendritic cells. Isoform 5 interacts with RANBP9. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: cytoplasm; plasma membrane; integral to membrane

Molecular Function: metal ion binding; MHC protein binding; carbohydrate binding; pattern recognition receptor activity

Biological Process: T cell activation; innate immune response; phagocytosis, recognition; carbohydrate mediated signaling; cell recognition; pattern recognition receptor signaling pathway; inflammatory response; defense response to protozoan

Disease: Aspergillosis, Susceptibility To; Candidiasis, Familial, 4