SDS-PAGE
LC20 recombinant protein
LC20 recombinant protein
Gene Names
Myl9; MLC20; RLC-C; Mylc2c; AI327049
Applications
Western Blot
Synonyms
LC20; LC20 recombinant protein; MYL9; MGC3505; MLC2; MRLC1; MYRL2
Host
E Coli
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Length
1131
Applicable Applications for LC20 recombinant protein
Kinase Assay, Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Mouse
Tag Information
GST tag
Expression System
E.coli
Source Note
Recombinant full-length mouse LC20 was expressed in E. coli cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for LC20 recombinant protein
Recombinant full-length mouse LC20 was expressed in E. coli cells using an N-terminal GST tag.
Scientific Background: LC20 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads (1). LC20 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for LC20 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the LC20 in conformational transitions of smooth muscle myosin (2).
Scientific Background: LC20 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads (1). LC20 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for LC20 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the LC20 in conformational transitions of smooth muscle myosin (2).
Product Categories/Family for LC20 recombinant protein
References
1. Wagner, P D. et al: Regulation of the actin-activated ATPase of aorta smooth muscle myosin. J Biol Chem. 1986 Jun 15;261(17):7778-83.
2. Trybus, K M. et al: The regulatory light chain is required for folding of smooth muscle myosin. J Biol Chem. 1988 Nov 5;263(31):16485-92.
2. Trybus, K M. et al: The regulatory light chain is required for folding of smooth muscle myosin. J Biol Chem. 1988 Nov 5;263(31):16485-92.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~45 kDa
NCBI Official Full Name
Mus musculus myosin, light polypeptide 9, regulatory, mRNA
NCBI Official Synonym Full Names
myosin, light polypeptide 9, regulatory
NCBI Official Symbol
Myl9
NCBI Official Synonym Symbols
MLC20; RLC-C; Mylc2c; AI327049
NCBI Protein Information
myosin regulatory light polypeptide 9; myosin regulatory light chain 9; myosin light chain, regulatory C; myosin regulatory light chain 2, smooth muscle isoform
UniProt Protein Name
Myosin regulatory light polypeptide 9
UniProt Gene Name
Myl9
UniProt Synonym Gene Names
Myrl2
UniProt Entry Name
MYL9_MOUSE
Uniprot Description
Function: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion
By similarity.
Subunit structure: Myosin is a hexamer of 2 heavy chains and 4 light chains
By similarity.
Post-translational modification: Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge
By similarity.
Miscellaneous: This chain binds calcium
By similarity.
Sequence similarities: Contains 3 EF-hand domains.
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Product Notes
The LC20 myl9 (Catalog #AAA515205) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||E.coli!!Source Note||Recombinant full-length mouse LC20 was expressed in E. coli cells. AAA Biotech's LC20 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the LC20 myl9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LC20, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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