Goat Protein C Inhibitor (PCI, also called PAI-3) Polyclonal Antibody

Goat anti-human Protein C Inhibitor (PCI), Peroxidase Conjugated IgG

Cat. Num. AAA512132

• Purity: Peroxidase conjugated IgG.; Vial containing ml of whole IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.2 mg.
• Synonyms: Protein C Inhibitor (PCI; also called PAI-3); Polyclonal Antibody; Goat anti-human Protein C Inhibitor (PCI); Peroxidase Conjugated IgG; anti-Protein C Inhibitor (PCI; also called PAI-3) antibody

• Host: Goat
• Clonality: Polyclonal
• Specificity: Prior to conjugation, this antibody was specific for Protein C Inhibitor as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Peroxidase conjugated IgG.; Vial containing ml of whole IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.2 mg.
• Form/Format: IgG-HRP conjugate as a clear, slightly red-brown liquid.
• Concentration: IgG-HRP concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 14. (varies by lot)

• Application Notes: Suitable as a source of peroxidase labeled antibodies to human Protein C Inhibitor.
• Immunogen: Human Protein C Inhibitor purified from plasma.
• Buffer: A buffered stabilizer solution containing 50% (v/v) glycerol.
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use. Avoid exposure to sodium azide as this is an inhibitor of peroxidase activity.

Related Product Information for anti-Protein C Inhibitor (PCI, also called PAI-3) antibody

Protein C Inhibitor (PCI), also known as Plasminogen Activator Inhibitor 3 (PAI3), is a member of the SERPIN family of proteinase inhibitors. It is produced in the liver as a single chain glycoprotein (mass of 57 kDa) and circulates in plasma at a concentration of 5 ug/ml (~90 nM). PCI is also found in urine in lower concentrations of 250 ng/mL (~0.4 nM). PCI is the primary inhibitor of activated Protein C (APC) in plasma but demonstrates a relatively broad specificity, also inhibiting thrombin, FXa, FXIa, kallikrein, tPA, urokinase, prostate specific antigen, acrosin, chymotrypsin and trypsin. The preferred enzyme target for PCI appears to be thrombin and this interaction is increased by more than 100 fold in the presence of thrombomodulin. Like ATIII and HCII, the inhibitory activity of PCI towards some of these enzymes is stimulated by high concentrations of heparin (5 U/ml) which can accelerate the rate of inactivation as much as 50 fold. Enzyme inhibition by PCI occurs through proteolytic cleavage at Arg354-Ser355 and subsequent rapid formation of a stable, inactive 1:1 enzyme-PCI complex. Interaction with APC results in an SDS-stable APC-PCI complex of 102 kDa1-4

References

1. Suzuki K; Protein C Inhibitor; Methods in Enzymology 222, pp 385- 399, 1993.
2. Cooper ST, Whinna HC, Jackson TP, Boyd JM, Church FC; Intermolecular Interactions between Protein C Inhibitor and Coagulation Proteases; Biochemistry: 34, pp. 12991-12997, 1995.
3. Heeb MJ, Espana F, Geiger M, Collen D, Stump DC, Griffin JH; Immunological Identity of Heparin-dependent Plasma & Urinary Protein C Inhibitor & Plasminogen Activator Inhibitor-3; JBC:262, pp 15813- 15816, 1987.
4. Rezaie AR, Copper ST, Church FC, Esmon CT; Protein C Inhibitor is a Potent Inhibitor of the Thrombin-Thrombomodulin Complex; JBC:270, pp 25336-25339, 1995.