Rabbit NCC Polyclonal Antibody | anti-NCC antibody

Anti-Phospho-Thr53 NCC Antibody

Cat. Num. AAA500073

• Gene Names: Slc12a3; NCC; TSC; NCCT; AI035291
• Reactivity: Guinea Pig, Hamster
• Applications: Western Blot
• Purity: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Synonyms: NCC; Polyclonal Antibody; Anti-Phospho-Thr53 NCC Antibody; anti-NCC antibody

• Host: Rabbit
• Reactivity: Guinea Pig, Hamster
• Clonality: Polyclonal
• Specificity: Specific for endogenous levels of the ~160 kDa NCC protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-Ptase.
• Purity/Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Form/Format: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 1002

• Applicable Applications for anti-NCC antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen Species: Mouse; Immunogen Information
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr53 of mouse NCC.
• Preparation and Storage: Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol.; Stable for at least 1 year at -20 degree C.

Western Blot (WB)

(Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda- Ptase, 1200 units for 30 min).)

Related Product Information for anti-NCC antibody

The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC at Thr53, Thr58, and Ser71 is an essential mediator of NCC function (Rosenbaek et al., 2014). NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015).

References

Feng X, Zhang Y, Shao N, Wang Y, Zhuang Z, Wu P, Lee MJ, Liu Y, Wang X, Zhuang J, Delpire E, Gu D, Cai H. (2015) Aldosterone modulates thiazide-sensitive sodium chloride cotransporter abundance via DUSP6-mediated ERK1/2 signaling pathway. Am J Physiol Renal Physiol. 308(10):F1119-27
Rosenbaek LL, Kortenoeven ML, Aroankins TS, Fenton RA. (2014) Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis. J Biol Chem. 289(19):13347-61

NCBI and Uniprot Product Information

• NCBI GI #: 328887916
• NCBI GeneID: 20497
• NCBI Accession #: NP_001192240.1
• NCBI GenBank Nucleotide #: NM_001205311.1
• UniProt Accession #: P59158
• Molecular Weight: ~160 kDa
• NCBI Official Full Name: solute carrier family 12 member 3 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 12, member 3
• NCBI Official Symbol: Slc12a3
• NCBI Official Synonym Symbols: NCC; TSC; NCCT; AI035291
• NCBI Protein Information: solute carrier family 12 member 3
• UniProt Protein Name: Solute carrier family 12 member 3
• UniProt Gene Name: Slc12a3
• UniProt Synonym Gene Names: Ncc; Tsc

Uniprot Description

Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption (). Receptor for the proinflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1 (PubMed:26099046).