Rabbit CK1Mt Polyclonal Antibody | anti-CKMT1A antibody

CK1Mt (Tyr153)

Cat. Num. AAA500054

• Gene Names: CKMT1A; CKMT1; U-MtCK; mia-CK
• Reactivity: Bovine, Canine, Guinea pig, Hamster, Non-Human primate, Rat, Rabbit, Sheep; Predicted: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified from Pooled Serum
• Synonyms: CK1Mt; Polyclonal Antibody; CK1Mt (Tyr153); anti-CKMT1A antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Guinea pig, Hamster, Non-Human primate, Rat, Rabbit, Sheep; Predicted: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified from Pooled Serum
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 417

• Applicable Applications for anti-CKMT1A antibody: Western Blot (WB)
• Application Notes: WB: 1:1,000
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr153 conjugated to KLH; Immunogen Species
• Purification Note: The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Preparation and Storage: Store at -20 degree C

Related Product Information for anti-CKMT1A antibody

Creatine kinase (CK1) plays an important role transferring phosphate groups from phosphocreatine to ADP in the cytosol of tissues having high, fluctuating energy demands like skeletal muscle, heart, and brain. Mitochondrial CK1, CK1Mt, has two isoforms, sarcomeric CK1Mt and ubiquitous CK1Mt. CK1Mt is localized between the MIM (mitochondrial inner membrane) and MOM (mitochondrial outer membrane) and bound to the cardiolipin-rich inner leaflet (Muller et al., 1985) and along the cristae membranes (Wegmann et al., 1991). CK1Mt exists in two forms; a homo dimer and an octamer consisting of four homodimers. The CK1Mt octamer forms permanent contact sites and maintains a complex structure including porin and ANT (adenine nucleotide translocase) within MIM and MOM (Speer et al, 2005). There have been several phospho-serine, threonine, and tyrosine sites identified within CK1Mt, the role of each one has yet to be determined.

Product Categories/Family for anti-CKMT1A antibody

Cardiovascular; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 66346730
• NCBI GeneID: 548596
• NCBI Accession #: NP_001015001.1
• NCBI GenBank Nucleotide #: NM_001015001.2
• UniProt Accession #: P12532; Q0VAM3; Q32NF6; Q53FC4; B4DIT8; B7ZA09
• Molecular Weight: ~46 kDa
• NCBI Official Full Name: creatine kinase U-type, mitochondrial isoform 1
• NCBI Official Synonym Full Names: creatine kinase, mitochondrial 1A
• NCBI Official Symbol: CKMT1A
• NCBI Official Synonym Symbols: CKMT1; U-MtCK; mia-CK
• NCBI Protein Information: creatine kinase U-type, mitochondrial
• UniProt Protein Name: Creatine kinase U-type, mitochondrial
• UniProt Gene Name: CKMT1A
• UniProt Synonym Gene Names: CKMT; Mia-CK; U-MtCK

NCBI Description

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.