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Western Blot (WB) (Left: Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~70k MAP2C/D protein. Right: Immunostaining of mixed neuron/glial cultures showing specific cytoplasmic labeling of dendrites and perikarya of neuronal cells in red with anti-MAP2C/D and astrocyte and fibroblast labelin(green) with anti-vimentin (cat # 2105-VIM).)

Mouse Microtubule Associated Protein 2 C/D Monoclonal Antibody | anti-MAP2 antibody

Anti-Microtubule Associated Protein 2 C/D (MAP2C/D)

Gene Names
MAP2; MAP2A; MAP2B; MAP2C
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Protein G Purified
Synonyms
Microtubule Associated Protein 2 C/D; Monoclonal Antibody; Anti-Microtubule Associated Protein 2 C/D (MAP2C/D); Microtubule Associated Protein 2 C/D (MAP2C/D); anti-MAP2 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
Labels the ~ 70k MAP2C/D isoforms as well as the ~280k MAP2A/B. The antibody will bind all known MAP2 isoforms.
Purity/Purification
Protein G Purified
Form/Format
100 ul in PBS + 50% glycerol and 5 mM sodium azide. Adequate amount of material to conduct 10 mini Western Blots.
Sequence Length
559
Applicable Applications for anti-MAP2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:5,000
IF/IHC: 1:2,500
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Left: Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~70k MAP2C/D protein. Right: Immunostaining of mixed neuron/glial cultures showing specific cytoplasmic labeling of dendrites and perikarya of neuronal cells in red with anti-MAP2C/D and astrocyte and fibroblast labelin(green) with anti-vimentin (cat # 2105-VIM).)

Western Blot (WB) (Left: Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~70k MAP2C/D protein. Right: Immunostaining of mixed neuron/glial cultures showing specific cytoplasmic labeling of dendrites and perikarya of neuronal cells in red with anti-MAP2C/D and astrocyte and fibroblast labelin(green) with anti-vimentin (cat # 2105-VIM).)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 70 kDa
NCBI Official Full Name
microtubule-associated protein 2 isoform 5
NCBI Official Synonym Full Names
microtubule associated protein 2
NCBI Official Symbol
MAP2
NCBI Official Synonym Symbols
MAP2A; MAP2B; MAP2C
NCBI Protein Information
microtubule-associated protein 2
UniProt Protein Name
Microtubule-associated protein 2
UniProt Gene Name
MAP2
UniProt Synonym Gene Names
MAP-2
UniProt Entry Name
MTAP2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Uniprot Description

MAP2: a member of the microtubule-associated protein (MAP) family. Its exact function is unknown but MAPs appear to stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Expressed at high levels in neural tissue and the prostate. Four alternatively spliced transcript variants have been reported.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q34-q35

Cellular Component: cytoplasm; microtubule; microtubule associated complex; neuron projection; nucleolus

Molecular Function: calmodulin binding; microtubule binding; protein binding; structural molecule activity

Biological Process: central nervous system neuron development; dendrite morphogenesis; microtubule bundle formation; microtubule cytoskeleton organization and biogenesis; neurite development

Research Articles on MAP2

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Product Notes

The MAP2 map2 (Catalog #AAA500052) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Microtubule Associated Protein 2 C/D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:5,000 IF/IHC: 1:2,500. Researchers should empirically determine the suitability of the MAP2 map2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Microtubule Associated Protein 2 C/D, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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