Typical Testing Data/Standard Curve (for reference only)
Fractalkine / CX3CL1 ELISA Kit | CX3CL1 elisa kit
Fractalkine / CX3CL1 (Mouse) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. An analyte-specific polyclonal antibody is pre-coated onto 96-well plates. The biotin conjugated second antibody is used as detection antibody. During the first incubation the standards and samples react with the analyte-specific pre-coated antibody. In a second incubation the biotin conjugated detection antibody completes the sandwich. After washing with wash buffer the TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The optical density of the yellow color is proportional to the analyte captured in plate. Read the O.D. absorbance at 450nm in a microtiterplate reader, and calculate the concentration of the analyte in the sample by taking into consideration the dilution factor of the sample!!Background/Introduction: Eotaxin, also known as Chemokine (C-C motif) ligand 11 (CCL11), is a small cytokine belonging to the CC chemokine family. These family members play an important role in the recruitment of leukocytes to inflammatory lesions. Eotaxin is a potent inducer of eosinophil chemotaxis leading to eosinophil migration in vitro and accumulation in vivo. It also interacts with CCR2 and CCR5 and can thus affect the responses of monocytes, which express both receptors. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. It has been found that eotaxin has high degree selectivity for its receptor, such that they are inactive on neutrophils and monocytes, which do not express CCR3.
NCBI and Uniprot Product Information
Uniprot Description
CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16q13
Cellular Component: cell projection; cell surface; extracellular region; extracellular space; integral to membrane; perinuclear region of cytoplasm; plasma membrane
Molecular Function: CCR chemokine receptor binding; chemokine activity; protein binding; receptor binding
Biological Process: aging; angiogenesis involved in wound healing; cell adhesion; chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; leukocyte adhesive activation; leukocyte chemotaxis; lymphocyte chemotaxis; macrophage chemotaxis; microglial cell activation; monocyte chemotaxis; negative regulation of cytokine secretion; negative regulation of vasoconstriction; neutrophil chemotaxis; platelet activation; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; positive regulation of GTPase activity; positive regulation of inflammatory response; positive regulation of neuroblast proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of transforming growth factor-beta1 production; regulation of inhibitory postsynaptic membrane potential; response to hypoxia
Research Articles on CX3CL1
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Product Notes
The CX3CL1 cx3cl1 (Catalog #AAA495370) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fractalkine / CX3CL1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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