Human Cyclic AMP ELISA Kit | CAMP elisa kit

Cyclic AMP (Direct) ELISA

Cat. Num. AAA494560

• Gene Names: CAMP; LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39
• Reactivity: Human
• Synonyms: Cyclic AMP; Cyclic AMP (Direct) ELISA; CAMP elisa kit

• Reactivity: Human
• Specificity: The cross reactivities for a number of related compounds were determined by diluting the cross reactants in the kit assay buffer at a concentration of ten times the high standard. These samples were then measured in the assay.
• Sequence Length: 170

• Samples: Cells, tissue, saliva, serum and culture supernatants
• Assay Type: Competitive
• Preparation and Storage: All components of this kit, except the conjugate and standard, are stable at 4 degree C until the kit's expiration date. The conjugate and standard should be stored at -20 degree C upon receipt.

Related Product Information for CAMP elisa kit

Intended Uses: Enzyme Immunoassay for the determination of cyclic AMP in celis, tissue, saliva, serum and culture supernatants. For research use only,

Principle of the Assay: Standards and unknowns are added to wells coated with a GxR IgG antibody. A blue solution of cAMP conjugated to alkaline phosphatase is then added, followed by a yellow solution of rabbit polyclonal antibody to cAMP.During a simultaneous incubation at room temperature the antibody binds, in a competitive manner, the cAMP in the unknown or conjugate. The plate is washed, leaving only bound cAMP. pNpp substrate solution is added. The substrate generates a yellow color when catalyzed by the alkaline phosphatase on the cAMP conjugate. Stop solution is added. The yellow color is read at 405nm. The amount of signal is indirectly proportional to the amount of cAMP in the unknown.

Background/Introduction: The cyclic AMP Complete Enzyme-linked Immunosorbent Assay (ELISA) kit is a competitive immunoassay for the determination of cyclic AMP in cells and tissue treated with O.lM HCI, in addition to culture supernatants, saliva, and serum. The optional acetylated assay format provides an approximate 10-fold increase in sensitivity and is ideal for unknowns with extremely low levels of cAMP. If expected levels of cAMP are unknown, the investigator may evaluate a few unknowns in the non-acetylated format in order to determine if higher sensitivity is required.

Product Categories/Family for CAMP elisa kit

Cytokines & Cell Signalling; Research Assays; Assay Categories

NCBI and Uniprot Product Information

• NCBI GI #: 49456877
• NCBI GeneID: 820
• UniProt Accession #: P49913; Q71SN9
• Molecular Weight: 19,301 Da
• NCBI Official Full Name: CAMP, partial
• NCBI Official Synonym Full Names: cathelicidin antimicrobial peptide
• NCBI Official Symbol: CAMP
• NCBI Official Synonym Symbols: LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39
• NCBI Protein Information: cathelicidin antimicrobial peptide; 18 kDa cationic antimicrobial protein
• UniProt Protein Name: Cathelicidin antimicrobial peptide
• Protein Family: Cyclic AMP receptor
• UniProt Gene Name: CAMP
• UniProt Synonym Gene Names: CAP18; FALL39; CAP-18; hCAP-18
• UniProt Entry Name: CAMP_HUMAN

NCBI Description

This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. [provided by RefSeq, Sep 2014]

Uniprot Description

CAMP: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. Belongs to the cathelicidin family.

Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted; Vesicle

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: extracellular space; specific granule; cell projection; cell wall; cytosol

Molecular Function: protein binding

Biological Process: interaction with host; defense response to Gram-positive bacterium; positive regulation of angiogenesis; innate immune response in mucosa; killing by host of symbiont cells; defense response to bacterium; positive regulation of cell proliferation; antibacterial humoral response; positive regulation of protein amino acid phosphorylation; defense response to Gram-negative bacterium