Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD115/M-CSFR antibody AFS98 (MBS488708) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of AFS98 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Rabbit anti-Mouse CD115/M-CSFR Monoclonal Antibody | anti-CD115/M-CSFR antibody
Anti-CD115/M-CSFR [AFS98]
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD115/M-CSFR antibody AFS98 (MBS488708) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of AFS98 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD115/M-CSFR antibody AFS98 (MBS488708) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of AFS98 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
This antibody was raised against CD115.
Note on publication: This article describes the generation and characterisation of the AFS98 antibody.
NCBI and Uniprot Product Information
Uniprot Description
CSFR: an oncogenic tyrosine kinase receptor for CSF-1 (M-CSF). Drives growth and development of monocytes. Binding of CSF-1 induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Two point mutations seen in 10-20% of patients with acute myeloid leukemia, chronic myelomonocytic leukemia or myelodysplasia. One mutation appears to be both somatic and germline, and disrupts Cbl binding and receptor turnover. v-fms lacks the Cbl binding site and causes feline leukemia. Mutations may also develop after chemotherapy for lymphoma. A distinct point mutation was found in some cases of hepatocellular carcinoma and related to increased expression, and another mutation was found in 2 of 40 patients with idiopathic myelofibrosis. Expression is elevated in breast tumors and cell lines, and expression in xenografts and transgenic mice has been correlated with xenograft growth and breast cancer development. Inhibitors: Ki-20227 and other Kit/PDGFR inhibitors.
Protein type: Protein kinase, TK; Oncoprotein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Kinase, protein; TK group; PDGFR family
Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; intracellular; receptor complex
Molecular Function: transferase activity; macrophage colony stimulating factor receptor activity; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; cytokine binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; ATP binding; protein kinase activity
Biological Process: regulation of bone resorption; phosphoinositide-mediated signaling; peptidyl-tyrosine phosphorylation; positive regulation of osteoclast differentiation; immune system process; cytokine and chemokine mediated signaling pathway; protein amino acid autophosphorylation; osteoclast differentiation; protein amino acid phosphorylation; positive regulation of tyrosine phosphorylation of Stat3 protein; cell proliferation; regulation of cell shape; positive regulation of cell proliferation; ruffle organization and biogenesis; innate immune response; hemopoiesis; positive regulation of protein amino acid phosphorylation; phosphatidylinositol metabolic process; inflammatory response; intercellular junction maintenance; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration
Research Articles on CD115/M-CSFR
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Product Notes
The CD115/M-CSFR csf1r (Catalog #AAA488708) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD115/M-CSFR [AFS98] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD115/M-CSFR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Blocking (BL), Western Blot (WB), Immunohistochemistry (IHC). AFS98 has been widely used in various flow cytometric analyses to label CD115 antigen. For instance, one group used it to detect monocytes in a mouse blood sample (Buschor et al., 2019). Similarly, Huo and colleagues (2017) utilised this antibody to identify monocytes in mouse blood in order to assess whether myelopoiesis was not a cause of the increase in the monocytes number. This antibody has been also successfully used to deplete macrophages (Ho et al., 2016). Finally, it was also utilised in the immunofluorescence staining of tissue sections prepared from 4% paraformaldehyde-fixed and decalcified bones (Dong et al., 2016). Researchers should empirically determine the suitability of the CD115/M-CSFR csf1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD115/M-CSFR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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