Western Blot (WB)
(Western Blot using anti-Podoplanin (MAP tag) antibody PMab-1 (MBS488581) Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (MBS488486) at 0.01ug/ml, 0.1ug/ml, 0.001ug/ml and 0.003ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Rat anti-Mouse Podoplanin Monoclonal Antibody | anti-PDPN antibody
Anti-Podoplanin (MAP tag) [PMab-1]
Gene Names
Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified antibody. Protein A affinity purified
Synonyms
Podoplanin; Monoclonal Antibody; Anti-Podoplanin (MAP tag) [PMab-1]; PDPN; Aggrus; Glycoprotein 38; Gp38; OTS-8; Ots8; PA2.26 antigen; T1-alpha; T1A; Transmembrane glycoprotein E11; anti-PDPN antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a, kappa
Clone Number
PMab-1
Specificity
This antibody is specific for amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin (PDPN). This antibody, therefore, possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), which is derived from the PLAG domain of murine podoplanin. PDPN is a type I transmembrane protein that interacts with the platelet receptor C-type lectin-like receptor-2 (CLEC-2). PDPN is expressed by a wide variety of cells, including podocytes, lymphatic endothelial cells and type I alveolar cells. PDPN is also upregulated on certain cancer cells, where it mediates tumour-induced platelet aggregation by binding to CLEC-2 on platelets. Chihara (2018) associated Podoplanin with a co-inhibitory receptor in T-cell activation along with PD-1, TIM-3, LAG-3 and TIGIT.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PDPN antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Blocking (BL), Epitope tag
Application Notes
This antibody binds to the PLAG domain of murine PDPN and is, therefore, able to block its interaction with C-type lectin-like receptor-2 (CLEC-2) (Yamada, 2017). This has a number of potential therapeutic applications, including the suppression of lymphangiogenesis and inflammatory macrophage infiltration at the site of wound healing in mouse corneal suture and ear section models (Maruyama et al, 2014), as well as suppression of transplant rejection. This antibody possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), and can, therefore, be used to detect and purify tagged proteins in a range of assay formats (Fujii et al, 2016). PMab-1 performs well in immunohistochemical analysis, and has been used to stain the mouse melanoma B16-F10 and mouse podoplanin-expressing CHO transfectants (Kaji et al, 2012). The use of this antibody in immunostaining of somatic tissues, including mouse lung lymphatic vessels, kidney glomeruli, pulmonary alveoli, pulmonary pleura and salivary gland myoepithelial cells, has been successfully performed on both paraffin-embedded sections and frozen sections (Kaji et al, 2012). The specificity of this antibody has been confirmed in Western blot analysis (Kaji et al, 2012), while Western blot analysis has also revealed that activation of LPS-induced inflammatory pathways, including the MAPK and NF-kappaB pathways, is affected by PMab-1 (Maruyama et al, 2014). The mouse-rat chimeric antibody mPMab-1 is derived from PMab-1 (Yamada et al, 2017).
PMID
23012488
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Western Blot (WB)
(Western Blot using anti-Podoplanin (MAP tag) antibody PMab-1 (MBS488581) Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (MBS488486) at 0.01ug/ml, 0.1ug/ml, 0.001ug/ml and 0.003ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-Podoplanin (MAP tag) antibody PMab-1 (MBS488581) Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (MBS488486) at 0.01ug/ml, 0.1ug/ml, 0.001ug/ml and 0.003ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-Podoplanin (MAP tag) antibody PMab-1 (MBS488581) NIH3T3 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of PMab-1 (MBS488581, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-Podoplanin (MAP tag) antibody PMab-1 (MBS488581) NIH3T3 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of PMab-1 (MBS488581, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-PDPN antibody
Recombinant monoclonal antibody to Podoplanin (MAP tag). Recombinant Platform with variable regions (i.e. specificity) from the hybridoma PMab-1.
This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.
This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.
Product Categories/Family for anti-PDPN antibody
References
Kaji et al Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin Acta Histochem. Cytochem. 45 (4): 227-237, 2012. doi:10.1267/ahc.12008
Note on publication: Describes the original generation and characterisation of this antibody.
Note on publication: Describes the original generation and characterisation of this antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,233 Da
NCBI Official Full Name
podoplanin isoform 2
NCBI Official Synonym Full Names
podoplanin
NCBI Official Symbol
Pdpn
NCBI Official Synonym Symbols
T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
NCBI Protein Information
podoplanin
UniProt Protein Name
Podoplanin
Protein Family
UniProt Gene Name
Pdpn
UniProt Synonym Gene Names
Gp38; Ots8; Gp38; T1A
Uniprot Description
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
Research Articles on PDPN
Similar Products
Product Notes
The PDPN pdpn (Catalog #AAA488582) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Podoplanin (MAP tag) [PMab-1] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Podoplanin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Blocking (BL), Epitope tag. This antibody binds to the PLAG domain of murine PDPN and is, therefore, able to block its interaction with C-type lectin-like receptor-2 (CLEC-2) (Yamada, 2017). This has a number of potential therapeutic applications, including the suppression of lymphangiogenesis and inflammatory macrophage infiltration at the site of wound healing in mouse corneal suture and ear section models (Maruyama et al, 2014), as well as suppression of transplant rejection. This antibody possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), and can, therefore, be used to detect and purify tagged proteins in a range of assay formats (Fujii et al, 2016). PMab-1 performs well in immunohistochemical analysis, and has been used to stain the mouse melanoma B16-F10 and mouse podoplanin-expressing CHO transfectants (Kaji et al, 2012). The use of this antibody in immunostaining of somatic tissues, including mouse lung lymphatic vessels, kidney glomeruli, pulmonary alveoli, pulmonary pleura and salivary gland myoepithelial cells, has been successfully performed on both paraffin-embedded sections and frozen sections (Kaji et al, 2012). The specificity of this antibody has been confirmed in Western blot analysis (Kaji et al, 2012), while Western blot analysis has also revealed that activation of LPS-induced inflammatory pathways, including the MAPK and NF-kappaB pathways, is affected by PMab-1 (Maruyama et al, 2014). The mouse-rat chimeric antibody mPMab-1 is derived from PMab-1 (Yamada et al, 2017). Researchers should empirically determine the suitability of the PDPN pdpn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Podoplanin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
