EDAR recombinant antibody

Anti-EDAR [EDAR12]

Cat. Num. AAA488352

• Gene Names: Edar; dl; ED3; ED5; ED1R; EDA3; EDA-A1R
• Reactivity: Chicken, Dog, Rat, Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Protein A Affinity Purified
• Synonyms: EDAR; Monoclonal Recombinant Antibody; Anti-EDAR [EDAR12]; ectodysplasin A receptor; Tumor necrosis factor receptor superfamily member EDAR; Anhidrotic ectodysplasin receptor 1; Downless; Ectodermal dysplasia receptor; Ectodysplasin-A receptor; EDAR recombinant antibody

• Reactivity: Chicken, Dog, Rat, Human, Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG, Kappa
• Clone Number: EDAR12
• Specificity: EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.
• Purity/Purification: Protein A Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 448

• Applicable Applications for EDAR recombinant antibody: Agonist, ELISA (EIA), Surface Plasmon Resonance (SPR), Western Blot (WB)
• Application Notes: EDAR12 can be used in surrogate reporter assay in which Fas-sensitive cells were transfected with a construct formed by fusing the ectodomain of mouse or human EDAR to the intracellular domain of Fas. Binding of EDAR12 resulted in the induction of apoptosis in these cells, confirming the agonisitc activity of the antibody. (EC50 (dose required to kill half of EDAR:Fas-expressing Jurkat cells) hEDAR:Fas ~ 5 ng/ml; EC50 mEDAR:Fas ~ 10 ng/ml-both were less active than DEA1-Fc). Tail-hair formation was also rescued after administration of EDAR3 to newborn EDA-deficient Tabby mice, with 0.35 mg/kg required for half-maximal tail-hair reversion. EDAR123 can also be used in ELISA and as a Fab fragment (generated by ficin digestion) in SPR experiments. EDAR can also be recognized by EDAR12 under both reducing (+DTT) and non-reducing conditions.
• Immunogen: EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Labeled Substance: None
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Related Product Information for EDAR recombinant antibody

Recombinant monoclonal antibody to EDAR. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma EDAR12.
EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.

Product Categories/Family for EDAR recombinant antibody

XLHED; X-Linked Hypohidrotic Ectodermal Dysplasia

References

Kowalczyk et al. Molecular and therapeutic characterization of antiectodysplasin A receptor (EDAR) agonist monoclonal antibodies. J Biol Chem. 2011 Sep 2; 286(35):30769 79.

NCBI and Uniprot Product Information

• NCBI GI #: 6753714
• NCBI GeneID: 13608
• NCBI Accession #: NP_034230.1
• NCBI GenBank Nucleotide #: NM_010100.3
• UniProt Accession #: Q9R187; Q9DC43
• Molecular Weight: 48,434 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member EDAR
• NCBI Official Synonym Full Names: ectodysplasin-A receptor
• NCBI Official Symbol: Edar
• NCBI Official Synonym Symbols: dl; ED3; ED5; ED1R; EDA3; EDA-A1R
• NCBI Protein Information: tumor necrosis factor receptor superfamily member EDAR
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member EDAR
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Edar
• UniProt Synonym Gene Names: Dl

Uniprot Description

Receptor for EDA isoform TAA, but not for EDA isoform TA-2 (). May mediate the activation of NF-kappa-B and JNK. May promote caspase-independent cell death.