NANS recombinant protein

NANS, 1-359aa, Human, His tag, E Coli

Cat. Num. AAA203797

• Gene Names: NANS; SAS; SEMDG; SEMDCG; HEL-S-100
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: NANS; 1-359aa; Human; His tag; E Coli; Sialic acid synthase; SAS; N acetylneuraminate synthase; NANS recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC AKFQKSELEF KFNRKALERP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR YAEEVGIFFT ASGMDEMAVE FLHELNVPFF KVGSGDTNNF PYLEKTAKKG RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC FLQCTSAYPL QPEDVNLRVI SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT LDKTWKGSDH SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV DNHGKKIKS
• Sequence Length: 359

• Applicable Applications for NANS recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for NANS recombinant protein

NANS, also known as SAS, is a 359 amino acid protein that contains one AFP (antifreeze proteins)-like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis. Recombinant human NANS protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for NANS recombinant protein

Metabolism

References

Milstein O., et al. (2008) J Biol Chem. 283 (49):34414-22.; Aldape MJ., et al. (2007) J Infect Dis. 195 (12):1838-45.;

NCBI and Uniprot Product Information

• NCBI GI #: 12056473
• NCBI GeneID: 54187
• NCBI Accession #: NP_061819
• NCBI GenBank Nucleotide #: NM_018946.3
• UniProt Accession #: Q9NR45; Q8WUV9; Q9BWS6; Q9NVD4; B2RE98
• Molecular Weight: 42.4 kDa (379aa) confirmed by MALDI-TOF
• NCBI Official Full Name: sialic acid synthase
• NCBI Official Synonym Full Names: N-acetylneuraminate synthase
• NCBI Official Symbol: NANS
• NCBI Official Synonym Symbols: SAS; SEMDG; SEMDCG; HEL-S-100
• NCBI Protein Information: sialic acid synthase
• UniProt Protein Name: Sialic acid synthase
• Protein Family: Sialic acid synthase
• UniProt Gene Name: NANS
• UniProt Synonym Gene Names: SAS

NCBI Description

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]

Uniprot Description

Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.