Western Blot (WB)
(Western blot analysis of extracts from MDA cells using coflin1/cofilin2 (Ab-88) Antibody.)
Rabbit anti-Human, Mouse coflin1/cofilin2 Polyclonal Antibody | anti-CFL1 antibody
Anti-coflin1/cofilin2 (Ab-88) Rabbit pAb
ICC: 1:100-1:200
IHC: 1:50-1:100
Western Blot (WB)
(Western blot analysis of extracts from MDA cells using coflin1/cofilin2 (Ab-88) Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from MDA cells using coflin1/cofilin2 (Ab-88) Antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using coflin1/cofilin2 (Ab-88) Antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using coflin1/cofilin2 (Ab-88) Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using coflin1/cofilin2 (Ab-88) Antibody (left) or the same antibody preincubated with blocking peptide (right).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using coflin1/cofilin2 (Ab-88) Antibody (left) or the same antibody preincubated with blocking peptide (right).)
UniPort Summary Function: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
Uniprot Description
Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: cytoplasm; extracellular matrix; extracellular space; focal adhesion; membrane; nucleus; vesicle
Molecular Function: actin filament binding; protein binding
Biological Process: actin cytoskeleton organization and biogenesis; actin filament depolymerization; cytoskeleton organization and biogenesis; negative regulation of apoptosis; regulation of cell morphogenesis; response to virus; Rho protein signal transduction
Research Articles on CFL1
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Product Notes
The CFL1 cfl1 (Catalog #AAA477102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-coflin1/cofilin2 (Ab-88) Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's coflin1/cofilin2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB: 1:500-1:1000 ICC: 1:100-1:200 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the CFL1 cfl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "coflin1/cofilin2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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