Rabbit GSK3alpha Polyclonal Antibody | anti-GSK3alpha antibody

Anti-GSK3alpha (Ab-21) Rabbit pAb

Cat. Num. AAA477061

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Synonyms: GSK3alpha; Polyclonal Antibody; Anti-GSK3alpha (Ab-21) Rabbit pAb; anti-GSK3alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/mL (varies by lot)
• Sequence Length: 483

• Applicable Applications for anti-GSK3alpha antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; ICC: 1:100-1:200; IHC: 1:50-1:100
• Immunogen: Peptide sequence around aa.19~23 (T-S-S-F-A) derived from Human GSK3alpha.
• Sensitivity: The antibody detects endogenous level of total GSK3alpha protein. and does not detect GSK-3beta protein.

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed MCF7 cells using GSK3alpha (Ab-21) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3alpha (Ab-21) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-GSK3alpha antibody

Entrez Summary: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
UniPort Summary Function: Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN

NCBI and Uniprot Product Information

• NCBI GI #: 49574532
• NCBI GeneID: 2931
• NCBI Accession #: NP_063937.2
• NCBI GenBank Nucleotide #: NM_019884.2
• UniProt Accession #: P49840; O14959
• Molecular Weight: 51
• NCBI Official Full Name: glycogen synthase kinase-3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: GSK3A
• NCBI Protein Information: glycogen synthase kinase-3 alpha
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• UniProt Gene Name: GSK3A
• UniProt Synonym Gene Names: GSK-3 alpha

NCBI Description

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]

Uniprot Description

GSK3A: a constitutively active proline-directed protein kinase of the GSK family. A negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubule. Phosphorylates and inactivates glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Generally requires primed phosphorylation sites C-terminal to the targeted serine or threonine residues in its substrate. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factor deprivation. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized b-catenin mutants that lack GSK3 phosphorylation sites.

Protein type: CMGC group; EC 2.7.11.1; EC 2.7.11.26; GSK family; GSK subfamily; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: beta-catenin destruction complex; cytosol

Molecular Function: protein binding; protein serine/threonine kinase activity; tau-protein kinase activity

Biological Process: cellular response to insulin stimulus; insulin receptor signaling pathway; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of TOR signaling pathway; positive regulation of cAMP biosynthetic process; positive regulation of heart contraction; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of systemic arterial blood pressure