Rabbit FUBP1 Polyclonal Antibody | anti-FUBP1 antibody

FUBP1 Rabbit pAb

Cat. Num. AAA4759788

• Gene Names: FUBP1; FBP; FUBP; hDH V
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: FUBP1; Polyclonal Antibody; FUBP1 Rabbit pAb; far upstream element binding protein 1; FBP; FUBP; hDH V; anti-FUBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.72mg/ml (varies by lot)

• Applicable Applications for anti-FUBP1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000; IHC-P: 1:25-1:50
• Immunogen: Synthetic peptide of human FUBP1
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of FUBP1 in Jurkat cell lysate using FUBP1 Rabbit pAb at dilution 1/650)

Immunohistochemistry (IHC)

(Immunohistochemistry of FUBP1 in paraffin-embedded Human gastric cancer tissue using FUBP1 Rabbit pAb at dilution 1/25. Predicted cell location: Nucleus)

Related Product Information for anti-FUBP1 antibody

The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 17402900
• NCBI GeneID: 8880
• NCBI Accession #: NP_003893.2
• NCBI GenBank Nucleotide #: NM_003902.4
• UniProt Accession #: Q96AE4; Q12828
• Molecular Weight: 68,605 Da
• NCBI Official Full Name: far upstream element-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: far upstream element (FUSE) binding protein 1
• NCBI Official Symbol: FUBP1
• NCBI Official Synonym Symbols: FBP; FUBP; hDH V
• NCBI Protein Information: far upstream element-binding protein 1; DNA helicase V
• UniProt Protein Name: Far upstream element-binding protein 1
• Protein Family: Far upstream element-binding protein
• UniProt Gene Name: FUBP1
• UniProt Synonym Gene Names: FBP; FUSE-binding protein 1; hDH V
• UniProt Entry Name: FUBP1_HUMAN

NCBI Description

The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

FBP1: Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; Motility/polarity/chemotaxis; RNA-binding; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; transcription factor activity; single-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter