Rabbit anti-Human, Mouse EXOSC4 Polyclonal Antibody | anti-EXOSC4 antibody

EXOSC4 Rabbit pAb

Cat. Num. AAA4759375

• Gene Names: EXOSC4; SKI6; p12A; RRP41; Ski6p; RRP41A; Rrp41p; hRrp41p
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: EXOSC4; Polyclonal Antibody; EXOSC4 Rabbit pAb; exosome component 4; SKI6; p12A; RRP41; Ski6p; RRP41A; Rrp41p; hRrp41p; anti-EXOSC4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.84mg/ml (varies by lot)

• Applicable Applications for anti-EXOSC4 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200
• Immunogen: Fusion protein of human EXOSC4
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of EXOSC4 in Human fetal liver tissue, RAW264.7, PC-3, 293T, LO2, Hela and Jurkat cell lysates in order using EXOSC4 Rabbit pAb at dilution 1/650)

Immunohistochemistry (IHC)

(Immunohistochemistry of EXOSC4 in paraffin-embedded Human thyroid cancer tissue using EXOSC4 Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm and Nucleus)

Related Product Information for anti-EXOSC4 antibody

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC4 binds to ARE-containing RNAs.

NCBI and Uniprot Product Information

• NCBI GI #: 9506689
• NCBI GeneID: 54512
• NCBI Accession #: NP_061910.1
• NCBI GenBank Nucleotide #: NM_019037.2
• UniProt Accession #: Q9NPD3
• Molecular Weight: 26,383 Da
• NCBI Official Full Name: exosome complex component RRP41
• NCBI Official Synonym Full Names: exosome component 4
• NCBI Official Symbol: EXOSC4
• NCBI Official Synonym Symbols: SKI6; p12A; RRP41; Ski6p; RRP41A; Rrp41p; hRrp41p
• NCBI Protein Information: exosome complex component RRP41; exosome complex exonuclease RRP41; exosome component Rrp41; ribosomal RNA-processing protein 41
• UniProt Protein Name: Exosome complex component RRP41
• UniProt Gene Name: EXOSC4
• UniProt Synonym Gene Names: RRP41; SKI6
• UniProt Entry Name: EXOS4_HUMAN

Uniprot Description

EXOSC4: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC4 binds to ARE-containing RNAs. Belongs to the RNase PH family.

Protein type: Nucleolus; EC 3.1.13.-; Ribonuclease

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm; nucleolus; exosome (RNase complex); cytosol; nucleus

Molecular Function: protein binding; 3'-5'-exoribonuclease activity; exoribonuclease activity; AU-rich element binding

Biological Process: maturation of 5.8S rRNA; gene expression; DNA deamination; defense response to virus; positive regulation of cell growth; rRNA processing; mRNA catabolic process, deadenylation-dependent decay