Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 30 ug, Lane 1-2: Hepg2 cells, Jurkat cells, Primary antibody: 860255(Dap3 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Rabbit anti-Human DAP3 Polyclonal Antibody | anti-DAP3 antibody
DAP3 Rabbit pAb
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
DAP3; Polyclonal Antibody; DAP3 Rabbit pAb; DAP-3; 4921514D13Rik; anti-DAP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-DAP3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide of mouse Dap3
Conjugation
Unconjugated
Molecular Function
28S ribosomal protein S29, mitochondrial is a protein that in humans is encoded by the DAP3 gene. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. DAP3 has been shown to interact with FADD, TNFRSF10A, Glucocorticoid receptor and Heat shock protein 90kDa alpha (cytosolic), member A1.
Modification
Unmodified
Cell Position
Mitochondrion
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 30 ug, Lane 1-2: Hepg2 cells, Jurkat cells, Primary antibody: 860255(Dap3 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 30 ug, Lane 1-2: Hepg2 cells, Jurkat cells, Primary antibody: 860255(Dap3 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 860255(Dap3 Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 860255(Dap3 Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 860255(Dap3 Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 860255(Dap3 Antibody) at dilution 1/20. (Original magnification: ×200))
Product Categories/Family for anti-DAP3 antibody
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
45kDa
UniProt Protein Name
28S ribosomal protein S29, mitochondrial
Protein Family
UniProt Gene Name
Dap3
UniProt Synonym Gene Names
Mrps29; MRP-S29; S29mt; DAP-3
UniProt Entry Name
RT29_MOUSE
Similar Products
Product Notes
The DAP3 dap3 (Catalog #AAA4758246) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAP3 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DAP3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the DAP3 dap3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
