Western Blot (WB)
(Western blot analysis of NFIB / NF1B2 expression in NIH/3T3 cell lysate.)
Rabbit NFIB/NF1B2 Polyclonal Antibody | anti-NFIB/NF1B2 antibody
NFIB/NF1B2 Rabbit pAb
Gene Names
NFIB; CTF; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity purified
Synonyms
NFIB/NF1B2; Polyclonal Antibody; NFIB/NF1B2 Rabbit pAb; CTF; HMGIC/NFIB; NF-I/B; NF1-B; NF1B; NF1B2; NFI-B; NFI-RED; Nfib; NFIB2; NFIB3; anti-NFIB/NF1B2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
NFIB/NF1B2 Antibody detects endogenous levels of total NFIB/NF1B2
Purity/Purification
Affinity purified
Form/Format
Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-NFIB/NF1B2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human NFIB/NF1B2
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at +4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of NFIB / NF1B2 expression in NIH/3T3 cell lysate.)
Western Blot (WB)
(Western blot analysis of NFIB / NF1B2 expression in NIH/3T3 cell lysate.)
Related Product Information for anti-NFIB/NF1B2 antibody
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Product Categories/Family for anti-NFIB/NF1B2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
420
NCBI Official Full Name
Nuclear factor 1 B-type
NCBI Official Synonym Full Names
nuclear factor I/B
NCBI Official Symbol
NFIB
NCBI Official Synonym Symbols
CTF; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
NCBI Protein Information
nuclear factor 1 B-type; nuclear factor 1/B; TGGCA-binding protein; CCAAT-box-binding transcription factor
UniProt Protein Name
Nuclear factor 1 B-type
UniProt Gene Name
NFIB
UniProt Synonym Gene Names
NF1-B; Nuclear factor 1/B; CTF; NF-I/B; NFI-B
UniProt Entry Name
NFIB_HUMAN
Uniprot Description
NFI-B: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 9p24.1
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: DNA binding; double-stranded DNA binding
Biological Process: transcription from RNA polymerase II promoter; pontine nucleus development; glial cell differentiation; anterior commissure morphogenesis; chondrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; DNA replication; negative regulation of DNA binding; hindbrain development
Research Articles on NFIB/NF1B2
Similar Products
Product Notes
The NFIB/NF1B2 nfib (Catalog #AAA4757263) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFIB/NF1B2 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NFIB/NF1B2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:50 FC/FACS: 1:50. Researchers should empirically determine the suitability of the NFIB/NF1B2 nfib for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFIB/NF1B2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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