Western Blot (WB)
(Western blot detection of Gli3 in 293 cell lysates using Gli3 Rabbit pAb(1:1000 diluted).Predicted band size:170kDa.Observed band size:200kDa.)
Rabbit anti-Human Gli3 Polyclonal Antibody | anti-GLI3 antibody
Gli3 Rabbit pAb
ICC/IF: 1:20
Western Blot (WB)
(Western blot detection of Gli3 in 293 cell lysates using Gli3 Rabbit pAb(1:1000 diluted).Predicted band size:170kDa.Observed band size:200kDa.)
Western Blot (WB)
(Western blot detection of Gli3 in 293 cell lysates using Gli3 Rabbit pAb(1:1000 diluted).Predicted band size:170kDa.Observed band size:200kDa.)
NCBI and Uniprot Product Information
Uniprot Description
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. Plays a role in limb and brain development ().
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Product Notes
The GLI3 gli3 (Catalog #AAA4756490) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Gli3 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Gli3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000 ICC/IF: 1:20. Researchers should empirically determine the suitability of the GLI3 gli3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gli3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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