Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot detection of S6 Ribosomal Protein in Ramos, Jurkat, C6, 3T3 and Hela cell lysates using S6 Ribosomal Protein mouse mAb (1:2000 diluted).Predicted band size:60KDa.Observed band size:60KDa.)

Mouse S6 Ribosomal Protein Monoclonal Antibody | anti-RPS6 antibody

Anti-S6 Ribosomal Protein Mouse mAb

Gene Names
RPS6; S6
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purified
Synonyms
S6 Ribosomal Protein; Monoclonal Antibody; Anti-S6 Ribosomal Protein Mouse mAb; 40SribosomalproteinS6; Air8; NP33; PhosphoproteinNP33; Pp30; RibosomalproteinS6; RPS6; rps6; RS6; RS6_HUMAN; S6; S6RibosomalProtein; anti-RPS6 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Monoclonal
Isotype
lgG1
Clone Number
7B10-E11-E11
Purity/Purification
Affinity Purified
Form/Format
Liquid in PBS containing 50% glycerol, 0.05% BSA, and 0.03% Proclin 300, pH 7.3
Sequence Length
249
Applicable Applications for anti-RPS6 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:2000
ICC: 1:200
Immunogen
Synthetic peptide derived from human S6 Ribosomal Protein.
Target
S6 Ribosomal Protein Mouse Monoclonal Antibody detects endogenous levels of total S6 ribosomal protein independent of phosphorylation.
Sequence Similarities
Belongs to the ribosomal protein S6e family.
Cellular Localization
Cytoplasm.
Preparation and Storage
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle

Western Blot (WB)

(Western blot detection of S6 Ribosomal Protein in Ramos, Jurkat, C6, 3T3 and Hela cell lysates using S6 Ribosomal Protein mouse mAb (1:2000 diluted).Predicted band size:60KDa.Observed band size:60KDa.)

Western Blot (WB) (Western blot detection of S6 Ribosomal Protein in Ramos, Jurkat, C6, 3T3 and Hela cell lysates using S6 Ribosomal Protein mouse mAb (1:2000 diluted).Predicted band size:60KDa.Observed band size:60KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-S6 Ribosomal Protein mouse mAb (dilution 1:200).)

Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-S6 Ribosomal Protein mouse mAb (dilution 1:200).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using S6 Ribosomal Protein Mouse mAb.)

Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using S6 Ribosomal Protein Mouse mAb.)

Western Blot (WB)

(Western blot detection of S6 Ribosomal Protein in CHO-K1, Raji, Raw264.7 and COS7 cell lysates using S6 Ribosomal Protein mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.)

Western Blot (WB) (Western blot detection of S6 Ribosomal Protein in CHO-K1, Raji, Raw264.7 and COS7 cell lysates using S6 Ribosomal Protein mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.)
Related Product Information for anti-RPS6 antibody
May play an important role in controlling cell growth and proliferation through the selective translation ofparticular classes of mRNA
Product Categories/Family for anti-RPS6 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32kDa
NCBI Official Full Name
40S ribosomal protein S6
NCBI Official Synonym Full Names
ribosomal protein S6
NCBI Official Symbol
RPS6
NCBI Official Synonym Symbols
S6
NCBI Protein Information
40S ribosomal protein S6
UniProt Protein Name
40S ribosomal protein S6
Protein Family
UniProt Gene Name
RPS6

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

S6: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Belongs to the ribosomal protein S6e family.

Protein type: Translation

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: cytoplasm; cytosol; dendrite; membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex; small ribosomal subunit

Molecular Function: protein binding; protein kinase binding

Biological Process: glucose homeostasis; mRNA catabolic process, nonsense-mediated decay; positive regulation of apoptosis; ribosomal small subunit biogenesis and assembly; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; TOR signaling pathway; translation; translational initiation; viral transcription

Research Articles on RPS6

Similar Products

Product Notes

The RPS6 rps6 (Catalog #AAA475585) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-S6 Ribosomal Protein Mouse mAb reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's S6 Ribosomal Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:2000 ICC: 1:200. Researchers should empirically determine the suitability of the RPS6 rps6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S6 Ribosomal Protein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.