Rabbit anti-Human VPS53 Polyclonal Antibody | anti-VPS53 antibody

VPS53 Rabbit pAb

Cat. Num. AAA4754529

• Gene Names: VPS53; HCCS1; PCH2E; hVps53L; pp13624
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: VPS53; Polyclonal Antibody; VPS53 Rabbit pAb; HCCS1; PCH2E; hVps53L; pp13624; anti-VPS53 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-VPS53 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000
• Immunogen: Fusion protein of human VPS53
• Conjugation: Unconjugated
• Molecular Function: This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.
• Modification: Unmodified
• Cell Position: Endosome, Golgi apparatus, Membrane
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: A172 cell, Primary antibody: 123546(VPS53 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 123546(VPS53 Antibody) at dilution 1/20. (Original magnification: ×200))

Product Categories/Family for anti-VPS53 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 13543951
• NCBI GeneID: 55275
• UniProt Accession #: Q5VIR6; Q8WYW3; Q9BRR2; Q9BY02; Q9NV25; A8K2S8; B3FH42
• Molecular Weight: 94,405 Da
• NCBI Official Full Name: Homo sapiens vacuolar protein sorting 53 homolog (S. cerevisiae), mRNA
• NCBI Official Synonym Full Names: VPS53, GARP complex subunit
• NCBI Official Symbol: VPS53
• NCBI Official Synonym Symbols: HCCS1; PCH2E; hVps53L; pp13624
• NCBI Protein Information: vacuolar protein sorting-associated protein 53 homolog
• UniProt Protein Name: Vacuolar protein sorting-associated protein 53 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS53
• UniProt Entry Name: VPS53_HUMAN

NCBI Description

This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS53: May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Belongs to the VPS53 family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; endosome membrane

Molecular Function: protein binding

Biological Process: protein transport; retrograde transport, endosome to Golgi

Disease: Pontocerebellar Hypoplasia, Type 2e