Rabbit anti-Human RRAGA Polyclonal Antibody | anti-RRAGA antibody

RRAGA Rabbit pAb

Cat. Num. AAA4754491

• Gene Names: RRAGA; FIP1; RAGA; FIP-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: RRAGA; Polyclonal Antibody; RRAGA Rabbit pAb; FIP1; RAGA; FIP-1; anti-RRAGA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-RRAGA antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Fusion protein of human RRAGA
• Conjugation: Unconjugated
• Molecular Function: Ras-related GTP-binding protein A is a protein that in humans is encoded by the RRAGAgene. Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death.
• Modification: Unmodified
• Cell Position: Cytoplasm, Lysosome, Nucleus
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hepg2 cells, hela cells, Primary antibody: 123065(RRAGA Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 123065(RRAGA Antibody) at dilution 1/25. (Original magnification: ×200))

Product Categories/Family for anti-RRAGA antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 13623626
• NCBI GeneID: 10670
• UniProt Accession #: Q7L523; O00290; Q15347; B2R7L1
• Molecular Weight: 36,566 Da
• NCBI Official Full Name: Homo sapiens Ras-related GTP binding A, mRNA
• NCBI Official Synonym Full Names: Ras related GTP binding A
• NCBI Official Symbol: RRAGA
• NCBI Official Synonym Symbols: FIP1; RAGA; FIP-1
• NCBI Protein Information: ras-related GTP-binding protein A
• UniProt Protein Name: Ras-related GTP-binding protein A
• UniProt Gene Name: RRAGA
• UniProt Entry Name: RRAGA_HUMAN

Uniprot Description

RRAGA: Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF- alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Can occur as a homodimer, or form a heterodimer with RRAGC or RRAGD in a sequence-independent manner. Binds GTP. The GTP-bound form of RRAGA interacts with NOL8. Interacts with adenovirus E3 14.7 kDa protein. Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain. Belongs to the GTR/RAG GTP-binding protein family.

Protein type: G protein regulator, misc.

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; lysosomal membrane; lysosome; cytoplasm; nucleus

Molecular Function: GTPase activity; protein binding; protein homodimerization activity; GDP binding; GTP binding; protein heterodimerization activity; phosphoprotein binding

Biological Process: cell death; metabolic process; apoptosis; virus-host interaction; positive regulation of cytolysis; regulation of autophagy; cellular response to starvation; positive regulation of TOR signaling pathway