Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse heart tissue, Mouse brain tissue, Primary antibody: 122744(NDUFA3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds)
Rabbit anti-Mouse NDUFA3 Polyclonal Antibody | anti-NDUFA3 antibody
NDUFA3 Rabbit pAb
Gene Names
NDUFA3; B9; CI-B9
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity purified
Synonyms
NDUFA3; Polyclonal Antibody; NDUFA3 Rabbit pAb; B9; CI-B9; anti-NDUFA3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-NDUFA3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:200-1:1000
Immunogen
Fusion protein of human NDUFA3
Conjugation
Unconjugated
Molecular Function
NDUFA3 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3), also known as B9, is one of about 45 subunits comprising Complex I of the oxidative phosphorylation electron transport chain. Consisting of 84 amino acids and belonging to the Complex I NDUFA3 subunit family, NDUFA3 functions as an accessory subunit of the multi-protein mitochondrial membrane respiratory chain NADH dehydrogenase complex (known as Complex I). Complex I plays an important role in the transfer of electrons from NADH to the respiratory chain, a process that is essential for cellular respiration.
Modification
Unmodified
Cell Position
Membrane, Mitochondrion, Mitochondrion inner membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse heart tissue, Mouse brain tissue, Primary antibody: 122744(NDUFA3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds)
Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse heart tissue, Mouse brain tissue, Primary antibody: 122744(NDUFA3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds)
Product Categories/Family for anti-NDUFA3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
9,279 Da
NCBI Official Full Name
Homo sapiens NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa, mRNA
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit A3
NCBI Official Symbol
NDUFA3
NCBI Official Synonym Symbols
B9; CI-B9
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
Protein Family
UniProt Gene Name
NDUFA3
UniProt Synonym Gene Names
CI-B9
UniProt Entry Name
NDUA3_HUMAN
Uniprot Description
NDUFA3: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA3 subunit family.
Protein type: Mitochondrial; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.42
Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane; mitochondrial respiratory chain complex I
Molecular Function: NADH dehydrogenase (ubiquinone) activity
Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone
Research Articles on NDUFA3
Similar Products
Product Notes
The NDUFA3 ndufa3 (Catalog #AAA4754461) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFA3 Rabbit pAb reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFA3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the NDUFA3 ndufa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
