Mouse anti-Human Prostatic Acid Phosphatase Monoclonal Antibody | anti-ACPP antibody

Prostatic Acid Phosphatase Mouse mAb

Cat. Num. AAA4753977

• Gene Names: ACPP; ACP3; 5'-NT; ACP-3
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Chromatography
• Synonyms: Prostatic Acid Phosphatase; Monoclonal Antibody; Prostatic Acid Phosphatase Mouse mAb; acid phosphatase; prostate; ACP3; 5'-NT; ACP-3; anti-ACPP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Specificity: This antibody detects endogenous levels of human Prostatic Acid Phosphatase(PSAP)
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ACPP antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-1:500
• Immunogen: Synthesized peptide derived from human Prostatic Acid Phosphatase(PSAP)
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Benign Prostatic Hyperplasia. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Benign Prostatic Hyperplasia. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Benign Prostatic Hyperplasia. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Benign Prostatic Hyperplasia. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Prostate. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Prostate. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Prostatic carcinoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Prostatic carcinoma. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

Related Product Information for anti-ACPP antibody

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.Isoform 2: the cellular form also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling.MiscellaneousUsed as a test tool for staging metastatic prostatic cancer.
This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]

Product Categories/Family for anti-ACPP antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 130730
• NCBI GeneID: 55
• UniProt Accession #: P15309; Q5FBY0; Q96KY0; Q96QK9; Q96QM0; D3DNC6
• Molecular Weight: 386
• NCBI Official Full Name: Prostatic acid phosphatase
• NCBI Official Synonym Full Names: acid phosphatase, prostate
• NCBI Official Symbol: ACPP
• NCBI Official Synonym Symbols: ACP3; 5'-NT; ACP-3
• NCBI Protein Information: prostatic acid phosphatase; TMPase; 5'-nucleotidase; ecto-5'-nucleotidase; thiamine monophosphatase; prostatic acid phosphotase
• UniProt Protein Name: Prostatic acid phosphatase
• Protein Family: Prostatic acid phosphatase
• UniProt Gene Name: ACPP
• UniProt Synonym Gene Names: PAP; 5'-NT; TMPase
• UniProt Entry Name: PPAP_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]

Uniprot Description

ACPP: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Belongs to the histidine acid phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin; Phosphatase; EC 3.1.3.5; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: multivesicular body; extracellular space; Golgi cisterna; apical part of cell; lysosomal membrane; plasma membrane; integral to membrane; vesicle membrane; intracellular; nucleus; secretory granule; filopodium

Molecular Function: 5'-nucleotidase activity; choline binding; identical protein binding; acid phosphatase activity; protein homodimerization activity; phosphoric monoester hydrolase activity; thiamin phosphate phosphatase activity

Biological Process: thiamin metabolic process; dephosphorylation; positive regulation of adenosine receptor signaling pathway; nucleotide metabolic process; regulation of sensory perception of pain; adenosine metabolic process; purine base metabolic process