Rabbit anti-Human MHC class I Monoclonal Antibody | anti-MHC antibody

MHC class I Rabbit mAb

Cat. Num. AAA4753493

• Gene Names: HLA-A; HLAA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Affinity purified
• Synonyms: MHC class I; Monoclonal Antibody; MHC class I Rabbit mAb; HLA class I histocompatibility; HLA A; HLA B; anti-MHC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Specificity: MHC class I Antibody detects endogenous levels of total MHC class I
• Purity/Purification: Affinity purified
• Form/Format: Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Applicable Applications for anti-MHC antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ICC/IF: 1:50-1:200; FC: 1:50
• Immunogen: A synthesized peptide derived from human MHC class I
• Conjugation: Unconjugated
• Modification: Unmodification
• Sequence Similarity: Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
• Subcellular Location: Membrane > Single-pass type I membrane protein
• Preparation and Storage: Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of MHC class I in Hela,A549 cell lysates using MHC class I Rabbit mAb(1:1000 diluted). Observed band size:41kDa.)

Related Product Information for anti-MHC antibody

Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind peptides and present them to T lymphocytes. HLA-A, -B and -C encode membrane anchored heavy chains which heterodimerize with a light chain (b-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-A, -B and -C alleles.

NCBI and Uniprot Product Information

• NCBI GI #: 1168209
• NCBI GeneID: 3105
• UniProt Accession #: P30450; P30443; P01889; P01891; P01892; P13746; P16188; P04439; P05534; P10314; P10316; P16189; P16190
• Molecular Weight: 41,062 Da
• NCBI Official Full Name: HLA class I histocompatibility antigen, A-26 alpha chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, A
• NCBI Official Symbol: HLA-A
• NCBI Official Synonym Symbols: HLAA
• NCBI Protein Information: HLA class I histocompatibility antigen, A-1 alpha chain
• UniProt Protein Name: HLA class I histocompatibility antigen, A-26 alpha chain
• Protein Family: Myosin
• UniProt Gene Name: HLA-A
• UniProt Synonym Gene Names: HLAA

NCBI Description

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.