Rabbit GNA13 Monoclonal Antibody | anti-GNA13 antibody

GNA13 Rabbit mAb

Cat. Num. AAA4752754

• Gene Names: GNA13; G13
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: GNA13; Monoclonal Antibody; GNA13 Rabbit mAb; G13; anti-GNA13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Specificity: Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level). Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile patients (at protein level).
• Purity/Purification: Affinity purified
• Form/Format: 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-GNA13 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:5000; IHC: 1:200
• Immunogen: A synthetic peptide of human G protein alpha 13
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cell membrane, Cytoplasm, Membrane, Nucleus
• Preparation and Storage: Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of GNA13 [KO Validated] in Hela,Jurkat cell lysates using GNA13 Rabbit mAb [KO Validated](1:500 diluted). Predicted band size:44kDa. Observed band size:44kDa.)

Related Product Information for anti-GNA13 antibody

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:15240885, PubMed:16787920, PubMed:16705036, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed:16787920, PubMed:16705036, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333).

NCBI and Uniprot Product Information

• NCBI GI #: 540344574
• NCBI GeneID: 10672
• NCBI Accession #: NP_001269354.1
• NCBI GenBank Nucleotide #: NM_001282425.1
• UniProt Accession #: Q14344; Q8TD70; B2R977; B7Z7R0; F5H1G8
• Molecular Weight: 33,287 Da
• NCBI Official Full Name: guanine nucleotide-binding protein subunit alpha-13 isoform 2
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), alpha 13
• NCBI Official Symbol: GNA13
• NCBI Official Synonym Symbols: G13
• NCBI Protein Information: guanine nucleotide-binding protein subunit alpha-13; G-protein subunit alpha-13; g alpha-13
• UniProt Protein Name: Guanine nucleotide-binding protein subunit alpha-13
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: GNA13
• UniProt Synonym Gene Names: G alpha-13; G-protein subunit alpha-13
• UniProt Entry Name: GNA13_HUMAN

Uniprot Description

G-alpha 13: a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Activates Rho. Protein kinase A blocks Rho activation by phosphorylation of G-alpha(13). Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Regulates cytoplasmic as well as nuclear signaling events such as activation of the Jun N-terminal kinase signaling module, Na+/H+ exchangers, focal adhesion assemblies, and transcriptional activation of specific primary response genes.

Protein type: G protein, heterotrimeric alpha G(12); G protein; G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 17q24.3

Cellular Component: focal adhesion; membrane; brush border membrane; plasma membrane; melanosome; heterotrimeric G-protein complex; nucleus

Molecular Function: GTPase activity; signal transducer activity; protein binding; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding; type 1 angiotensin receptor binding; D5 dopamine receptor binding

Biological Process: phospholipase D activation; platelet activation; metabolic process; in utero embryonic development; signal transduction; G-protein signaling, adenylate cyclase activating pathway; regulation of cell migration; Rho protein signal transduction; patterning of blood vessels; regulation of cell shape; elevation of cytosolic calcium ion concentration; blood coagulation; cell differentiation; cell motility