Western Blot (WB)
(Western blot detection of SMARCC1/BAF155 in K562,C6,Hela cell lysates using SMARCC1/BAF155 Rabbit pAb(1:1000 diluted). Predicted band size:123kDa. Observed band size:155kDa.)
Rabbit anti-Human, Rat SMARCC1/BAF155 Polyclonal Antibody | anti-SMARCC1 antibody
SMARCC1/BAF155 Rabbit pAb
Gene Names
SMARCC1; Rsc8; SRG3; SWI3; BAF155; CRACC1
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
SMARCC1/BAF155; Polyclonal Antibody; SMARCC1/BAF155 Rabbit pAb; SWI/SNF complex subunit SMARCC1; Rsc8; SRG3; SWI3; BAF155; CRACC1; anti-SMARCC1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. Gene expression databases
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-SMARCC1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000-1:5000
ICC/IF: 1:50-1:200
FC/FACS: 1:20
IP: 1:20-1:50
ICC/IF: 1:50-1:200
FC/FACS: 1:20
IP: 1:20-1:50
Immunogen
A synthetic peptide of human SMARCC1
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Cytoplasm, Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot detection of SMARCC1/BAF155 in K562,C6,Hela cell lysates using SMARCC1/BAF155 Rabbit pAb(1:1000 diluted). Predicted band size:123kDa. Observed band size:155kDa.)
Western Blot (WB)
(Western blot detection of SMARCC1/BAF155 in K562,C6,Hela cell lysates using SMARCC1/BAF155 Rabbit pAb(1:1000 diluted). Predicted band size:123kDa. Observed band size:155kDa.)
Related Product Information for anti-SMARCC1 antibody
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq, Jul 2008]
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex (PubMed:10078207, PubMed:29374058). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex (PubMed:10078207, PubMed:29374058). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122,867 Da
NCBI Official Full Name
SWI/SNF complex subunit SMARCC1
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
NCBI Official Symbol
SMARCC1
NCBI Official Synonym Symbols
Rsc8; SRG3; SWI3; BAF155; CRACC1
NCBI Protein Information
SWI/SNF complex subunit SMARCC1; BRG1-associated factor 155; SWI/SNF complex 155 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1; chromatin remodeling complex BAF155 subunit; mammalian chromatin r
UniProt Protein Name
SWI/SNF complex subunit SMARCC1
Protein Family
UniProt Gene Name
SMARCC1
UniProt Synonym Gene Names
BAF155; BAF155
UniProt Entry Name
SMRC1_HUMAN
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Product Notes
The SMARCC1 smarcc1 (Catalog #AAA4752266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMARCC1/BAF155 Rabbit pAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCC1/BAF155 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000-1:5000 ICC/IF: 1:50-1:200 FC/FACS: 1:20 IP: 1:20-1:50. Researchers should empirically determine the suitability of the SMARCC1 smarcc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCC1/BAF155, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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