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Western Blot (WB) (Western blot detection of GTF2H1 in Hela, Hela NE and Jurkat cell lysates using GTF2H1 mouse mAb (1:300 diluted).Predicted band size: 62KDa.Observed band size: 62KDa.)

anti-Human GTF2H1 Monoclonal Antibody | anti-GTF2H1 antibody

Anti-GTF2H1 Mouse mAb

Gene Names
GTF2H1; P62; BTF2; TFB1; TFIIH
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry
Purity
Affinity Purified
Synonyms
GTF2H1; Monoclonal Antibody; Anti-GTF2H1 Mouse mAb; Basic transcription factor 2 62 kDa subunit; Basic transcription factor 62 kDa subunit; BTF 2; BTF2; BTF2 p62; General transcription factor IIH polypeptide 1; General transcription factor IIH subunit 1; General transcription factor IIH; polypeptide 1; 62kDa; gtf2h1; TF2H1; TF2H1_HUMAN; TFB1; TFIIH; TFIIH basal transcription factor complex p62 subunit; anti-GTF2H1 antibody
Ordering
For Research Use Only!
Host
Mouse
Source: Human
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3E8-D3-H8
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)with 0.2% sodium azide, 0.1mg/mlBSA and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
548
Applicable Applications for anti-GTF2H1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
WB: 1:300
ICC: 1:50
Immunogen
Purified recombinant human GTF2H1protein fragments expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of GTF2H1 and does not cross-react with related proteins.
Sequence Similarities
Contains 2 BSD domains
Subunit Structure
One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with PUF60.
Subcellular Location
Nucleus

Western Blot (WB)

(Western blot detection of GTF2H1 in Hela, Hela NE and Jurkat cell lysates using GTF2H1 mouse mAb (1:300 diluted).Predicted band size: 62KDa.Observed band size: 62KDa.)

Western Blot (WB) (Western blot detection of GTF2H1 in Hela, Hela NE and Jurkat cell lysates using GTF2H1 mouse mAb (1:300 diluted).Predicted band size: 62KDa.Observed band size: 62KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using GTF2H1 mouse mAb (dilution 1:50).)

Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using GTF2H1 mouse mAb (dilution 1:50).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using GTF2H1 mouse mAb.)

Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using GTF2H1 mouse mAb.)
Related Product Information for anti-GTF2H1 antibody
UniPort Summary Function: Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62
NCBI Official Full Name
general transcription factor IIH subunit 1
NCBI Official Synonym Full Names
general transcription factor IIH subunit 1
NCBI Official Symbol
GTF2H1
NCBI Official Synonym Symbols
P62; BTF2; TFB1; TFIIH
NCBI Protein Information
general transcription factor IIH subunit 1
UniProt Protein Name
General transcription factor IIH subunit 1
UniProt Gene Name
GTF2H1
UniProt Synonym Gene Names
BTF2; BTF2 p62

Uniprot Description

GTF2H1: Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: holo TFIIH complex; nucleoplasm

Molecular Function: DNA-dependent ATPase activity; protein binding; protein kinase activity; RNA polymerase subunit kinase activity

Biological Process: DNA repair; mRNA capping; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; positive regulation of transcription from RNA polymerase II promoter; regulation of cyclin-dependent protein kinase activity; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; termination of RNA polymerase I transcription; transcription from RNA polymerase I promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair

Research Articles on GTF2H1

Similar Products

Product Notes

The GTF2H1 gtf2h1 (Catalog #AAA475091) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-GTF2H1 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2H1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). WB: 1:300 ICC: 1:50. Researchers should empirically determine the suitability of the GTF2H1 gtf2h1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2H1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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