Rabbit NLRC4 Polyclonal Antibody | anti-NLRC4 antibody

NLRC4 (a.a. 525-538) Antibody

Cat. Num. AAA474481

• Gene Names: Nlrc4; CLAN; IPAF; CLAN1; CLANA; CLANB; CLANC; CLAND; Card12; 9530011P19Rik
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Synonyms: NLRC4; Polyclonal Antibody; NLRC4 (a.a. 525-538) Antibody; NLRC4 (a.a. 525-538); anti-NLRC4 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: The antibody was affinity purified using NLRC4 (a.a. 525-538) peptide. This antibody antibody detects a 110kDa* protein on SDS-PAGE immunoblots of PMA-differentiated THP1 human macrophages. In addition, the antibody detects a mouse recombinant phospho-NLRC4 (Ser-533) protein fragment.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-NLRC4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000
• Immunogen: NLRC4 (a.a. 525-538) synthetic peptide (coupled to carrier) corresponding to amino acids in mouse NLRC4. This sequence has high homology to human and rat NLRC4, and is not homologous to sequences in other NLR family members.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot image of mouse recombinant NLRC4 (Ser-533) phosphorylated peptide (lanes 1 & 4) and dephosphorylated peptide (lanes 2 & 5), as well as human PMA-differentiated THP1 cells (lanes 3 & 6). The blots were probed with rabbit polyclonals anti-NLRC4 (a.a. 525-538) (lanes 1-3) and anti-NLRC4 (Ser-533) phospho-specific (lanes 4-6).)

Related Product Information for anti-NLRC4 antibody

The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family is a diverse family of cytoplasmic innate immune receptors that are involved in recognition of pathogen-associated molecular patterns. NLRs are important for pathogen sensing, transcriptional activation of proinflammatory cytokines and activation of inflammatory caspases. NLRC4 (IPAF, CARD12) forms the inflammasome that responds to bacterial flagellin. This inflammasome is activated by NLRC4 oligomerization, NAIP protein binding, and activation of caspase-1 leading to pyroptosis. NLRC4 is phosphorylated on Ser-533 by PKCdelta following infection of macrophages with S. typhimurium. Mutant forms of NLRC4 demonstrate that an unphosphorylatable form (S533A) does not activate caspase-1 and pyroptosis in response to S. typhimurium, while a phosphomimetic NLRC4 (S533D) mutant causes rapid macrophage pyroptosis without infection. Thus, PKCdelta phosphorylation of NLRC4 (S533) may be a critical event in inflammasome activation and host innate immunity.

References

Qu, Y. et al. (2012) Nature 490:539.
Elinav, E. et al. (2011) Immunity 34:665.
Poyet, J.L. et al. (2001) J Biol Chem 276:28309.

NCBI and Uniprot Product Information

• NCBI GI #: 77736546
• NCBI GeneID: 268973
• NCBI Accession #: NP_001028539.1
• NCBI GenBank Nucleotide #: NM_001033367.3
• UniProt Accession #: Q3UP24; Q3TAU8
• Molecular Weight: 116,749 Da
• NCBI Official Full Name: NLR family CARD domain-containing protein 4
• NCBI Official Synonym Full Names: NLR family, CARD domain containing 4
• NCBI Official Symbol: Nlrc4
• NCBI Official Synonym Symbols: CLAN; IPAF; CLAN1; CLANA; CLANB; CLANC; CLAND; Card12; 9530011P19Rik
• NCBI Protein Information: NLR family CARD domain-containing protein 4
• UniProt Protein Name: NLR family CARD domain-containing protein 4
• Protein Family: NLR family CARD domain-containing protein
• UniProt Gene Name: Nlrc4
• UniProt Synonym Gene Names: Card12; Ipaf; Ipaf
• UniProt Entry Name: NLRC4_MOUSE

Uniprot Description

NLRC4: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Cellular Component: cytoplasm; cytosol; nucleus; spindle microtubule

Molecular Function: ATP binding; cysteine protease inhibitor activity; identical protein binding; nucleotide binding; protein homodimerization activity; ubiquitin-protein ligase activity

Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; apoptosis; caspase activation; defense response to bacterium; detection of bacterium; immune system process; inflammatory response; innate immune response; interleukin-1 beta secretion; positive regulation of apoptosis; protein homooligomerization; regulation of apoptosis; regulation of caspase activity; regulation of signal transduction