Western Blot (WB)
(Native western blot analysis of human MDA-MB-231 breast cancer cell lysate (lanes 1 & 2),human recombinant CD55 extracellular region protein (lane 3), and CD44 extracellular region protein (lane 4). The blots were probed with mouse monoclonal anti-CD55 (CM0331) at 1:1000 (lane 1) and 1:4000 (lanes 2, 3, and 4).)
Mouse anti-Human CD55 Monoclonal Antibody | anti-CD55 antibody
CD55 (Extracellular region) M033 Antibody
ICC: 1:100
IP: 1:50
WB: 1:1000
Western Blot (WB)
(Native western blot analysis of human MDA-MB-231 breast cancer cell lysate (lanes 1 & 2),human recombinant CD55 extracellular region protein (lane 3), and CD44 extracellular region protein (lane 4). The blots were probed with mouse monoclonal anti-CD55 (CM0331) at 1:1000 (lane 1) and 1:4000 (lanes 2, 3, and 4).)
Western Blot (WB)
(Native western blot analysis of human MDA-MB-231 breast cancer cell lysate (lanes 1 & 2),human recombinant CD55 extracellular region protein (lane 3), and CD44 extracellular region protein (lane 4). The blots were probed with mouse monoclonal anti-CD55 (CM0331) at 1:1000 (lane 1) and 1:4000 (lanes 2, 3, and 4).)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0331). The antibody was detected using goat anti-mouse DyLight 594.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0331). The antibody was detected using goat anti-mouse DyLight 594.)
Standard Curve
(Representative Standard Curve using mouse monoclonal anti-CD55 (CM0331) for ELISA capture of human recombinant CD55 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
Standard Curve
(Representative Standard Curve using mouse monoclonal anti-CD55 (CM0331) for ELISA capture of human recombinant CD55 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
Mikesch JH et al.(2006) Cell Oncol. 28(5-6):223.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, GPI anchor; Cell surface
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: cell surface; integral to plasma membrane; extracellular region; plasma membrane; lipid raft
Molecular Function: viral receptor activity; protein binding; lipid binding
Biological Process: respiratory burst; entry of virus into host cell; elevation of cytosolic calcium ion concentration; negative regulation of complement activation; regulation of complement activation; innate immune response; regulation of lipopolysaccharide-mediated signaling pathway; complement activation, classical pathway
Disease: Blood Group, Cromer System
Research Articles on CD55
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Product Notes
The CD55 cd55 (Catalog #AAA474232) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD55 (Extracellular region) M033 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD55 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA)Cap, Immunocytochemistry (ICC), Immunoprecipitation (IP). ELISA (Cap): 1:100 ICC: 1:100 IP: 1:50 WB: 1:1000. Researchers should empirically determine the suitability of the CD55 cd55 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD55, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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