Argonaute 2 Phospho-Regulation Antibody Sampler Kit
Argonaute 2 Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
Argonaute 2 Phospho-Regulation; Argonaute2K; Argonaute 2 Phospho-Regulation antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Primary antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for Argonaute 2 Phospho-Regulation antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C. Stable for 1 year.
Related Product Information for Argonaute 2 Phospho-Regulation antibody sampler kit
Kit Summary: The argonaute 2 (Ago2) phospho-regulation antibody sampler kit can be used to detect phosphorylation of Ser-389 andTyr-393 relative to total Ago2 expression levels. The kit includes rabbit polyclonal phospho-specific antibodies to Ser-389and Tyr-393, as well as rat monoclonal and rabbit polyclonal antibodies for detection of total Ago2. The kit also includesanti-Rabbit Light Chain specific:HRP and anti-Rat Ig:HRP secondary reagents for detection of antibodies in western blot, ELISA, or immunocytochemistry.
Background: Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interactingRNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in manycellular processes such as development, differentiation, and stress response. These small RNAs function together withcomplexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation orstability. Among the most important components in these complexes are argonaute proteins. There are four members inthe mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonauteproteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNAturnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFRphosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylatesAgo2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.
Background: Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interactingRNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in manycellular processes such as development, differentiation, and stress response. These small RNAs function together withcomplexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation orstability. Among the most important components in these complexes are argonaute proteins. There are four members inthe mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonauteproteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNAturnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFRphosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylatesAgo2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.
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Product Notes
The Human, Rat, Mouse Argonaute 2 Phospho-Regulation (Catalog #AAA474157) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474157 Antibody Sampler Kit recognizes Human, Rat, Mouse Argonaute 2 Phospho-Regulation. AAA Biotech's Argonaute 2 Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the Argonaute 2 Phospho-Regulation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Argonaute 2 Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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