Rabbit Argonaute 2 Polyclonal Antibody | anti-AGO2 antibody

Argonaute 2 (a.a. 389-398) Antibody

Cat. Num. AAA474190

• Gene Names: AGO2; Q10; EIF2C2
• Reactivity: Human, Rat, Mouse, Chicken, Fish
• Applications: Western Blot, ELISA, Immunocytochemistry
• Synonyms: Argonaute 2; Polyclonal Antibody; Argonaute 2 (a.a. 389-398) Antibody; Argonaute 2 (a.a. 389-398); Argonaute2 a389; anti-AGO2 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken, Fish
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using Ago2 (a.a. 389-398) peptide (without carrier). The antibody detects a 97kDa* protein corresponding to the apparent molecular mass of Ago2 on SDS-PAGE immunoblots of human A431 cells and mouse recombinant Ago2 full length protein.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-AGO2 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; WB: 1:1000; ICC: 1:100
• Immunogen: Ago2 (a.a. 389-398) synthetic peptide (coupled to KLH) corresponding to amino acid 389 to 398 in human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of mouse recombinant Ago2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2 (AP5281) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Ser-387) phospho-specific antibody (lanes 3 & 4).)

Related Product Information for anti-AGO2 antibody

Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.

References

Shen, J. et al. (2013) Nature 497:383.
Horman, S.R. et al. (2013) Mol Cell 50:356.
Filipowicz, W. et al. (2008) Nat Rev Genet. 9:102.
Peters, L. & Meister, G. (2007) Mol Cell 26: 611.

NCBI and Uniprot Product Information

• NCBI GI #: 229463006
• NCBI GeneID: 27161
• UniProt Accession #: Q9UKV8; Q8TCZ5; Q8WV58; Q96ID1
• Molecular Weight: 859
• NCBI Official Full Name: Protein argonaute-2
• NCBI Official Synonym Full Names: argonaute RISC catalytic component 2
• NCBI Official Symbol: AGO2
• NCBI Official Synonym Symbols: Q10; EIF2C2
• NCBI Protein Information: protein argonaute-2; PPD; hAgo2; eIF2C 2; eIF-2C 2; argonaute2; argonaute 2; protein slicer; PAZ Piwi domain protein; eukaryotic translation initiation factor 2C, 2
• UniProt Protein Name: Protein argonaute-2
• Protein Family: Protein argonaute
• UniProt Gene Name: AGO2
• UniProt Synonym Gene Names: hAgo2
• UniProt Entry Name: AGO2_HUMAN

NCBI Description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: RNA processing; RNA-binding; EC 3.1.26.n2

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: nucleoplasm; polysome; mRNA cap complex; membrane; cytoplasm; ribonucleoprotein complex; cytosol

Molecular Function: mRNA binding; endoribonuclease activity; protein binding; miRNA binding; siRNA binding; translation initiation factor activity; metal ion binding; RNA 7-methylguanosine cap binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of translational initiation; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; RNA interference, siRNA loading onto RISC; translation; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, negative regulation of translation; post-embryonic development; regulation of transcription, DNA-dependent; pre-microRNA processing; translational initiation; innate immune response; RNA-mediated gene silencing; gene expression; posttranscriptional gene silencing