Rabbit VE-Cadherin Polyclonal Antibody | anti-CDH5 antibody

VE-Cadherin (a.a. 770-781)

Cat. Num. AAA472065

• Gene Names: CDH5; 7B4; CD144; FLJ17376
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified
• Synonyms: VE-Cadherin; Polyclonal Antibody; VE-Cadherin (a.a. 770-781); Cadherin-5; anti-CDH5 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified with VE-cadherin (a.a.770-781) peptide. The purified antibody detects a 140 kDa* band corresponding to VE-cadherin in western blots of human endothelial cells, and this reactivity can be specifically blocked using VE-cadherin peptide (CX2235).
• Purity/Purification: Affinity Purified
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-CDH5 antibody: ELISA, Western Blot
• Application Notes: ELISA: 1:2000; Western Blot: 1:1000
• Immunogen: VE-Cadherin synthetic peptide (coupled to carrier protein) corresponds to amino acids 770 to 781 in human VE-cadherin. This sequence has significant homology to the conserved site in rat and mouse, and has less than 50% homology with other cadherins.
• Preparation and Storage: Store at -20 degree C. Do not aliquot. Stable for 1 year.

Testing Data

Related Product Information for anti-CDH5 antibody

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VEcadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with beta- or gamma-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.

References

• Potter M.D. et al. (2005) J Biol. Chem. 280(36):31906
• Baumeister U. et al. (2005) EMBOJ 24:1686
• Wallez Y. et al. (2007) Oncogene 26:1067

NCBI and Uniprot Product Information

• NCBI GI #: 599834
• NCBI GeneID: 1003
• UniProt Accession #: P33151; Q4VAI4; Q4VAI5; Q4VAI6; Q59EA3
• Molecular Weight: 87,528 Da
• NCBI Official Full Name: VE-cadherin
• NCBI Official Synonym Full Names: cadherin 5, type 2 (vascular endothelium)
• NCBI Official Symbol: CDH5
• NCBI Official Synonym Symbols: 7B4; CD144; FLJ17376
• NCBI Protein Information: cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; OTTHUMP00000174777; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium)
• UniProt Protein Name: Cadherin-5
• Protein Family: Cadherin
• UniProt Gene Name: CDH5
• UniProt Entry Name: CADH5_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]

Uniprot Description

Function: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Ref.9

Subunit structure: Interacts via cadherin 5 domain with PTPRB

By similarity. Interacts with TRPC4. Interacts with KRIT1. Ref.8 Ref.9

Subcellular location: Cell junction. Cell membrane; Single-pass type I membrane protein

Probable. Note: Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions. Ref.9

Tissue specificity: Endothelial tissues and brain.

Post-translational modification: Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB

By similarity. Ref.7

Sequence similarities: Contains 5 cadherin domains.